Author(s):
Carvalho-Santos, Zita ; Machado, Pedro ; Alvarez-Martins, Inês ; Gouveia, Susana M. ; Jana, Swadhin C. ; Duarte, Paulo ; Amado, Tiago ; Branco, Pedro ; Freitas, Micael C. ; Silva, Sara T.N. ; Antony, Claude ; Bandeiras, Tiago M. ; Bettencourt-Dias, Mónica
Date: 2012
Persistent ID: http://hdl.handle.net/10400.7/835
Origin: ARCA - Access to Research and Communication Annals
Project/scholarship:
info:eu-repo/grantAgreement/FCT/3599-PPCDT/105602/PT
;
info:eu-repo/grantAgreement/EC/FP7/261344/EU;
Subject(s): Animals; Drosophila Proteins; Drosophila melanogaster; Flagella; Male; Microscopy, Electron, Transmission; Microtubule-Associated Proteins; Microtubules; Protein Binding; Spermatogenesis; Cilia
Description
The deposited article is a post-print version and has been submitted to peer review.
The deposited article is a pre-print version
This deposit is composed by the main article and the supplementary materials are present in the publisher's page in the following link: https://ars.els-cdn.com/content/image/1-s2.0-S1534580712002511-mmc1.pdf
Cilia and flagella are involved in a variety of processes and human diseases, including ciliopathies and sterility. Their motility is often controlled by a central microtubule (MT) pair localized within the ciliary MT-based skeleton, the axoneme. We characterized the formation of the motility apparatus in detail in Drosophila spermatogenesis. We show that assembly of the central MT pair starts prior to the meiotic divisions, with nucleation of a singlet MT within the basal body of a small cilium, and that the second MT of the pair only assembles much later, upon flagella formation. BLD10/CEP135, a conserved player in centriole and flagella biogenesis, can bind and stabilize MTs and is required for the early steps of central MT pair formation. This work describes a genetically tractable system to study motile cilia formation and provides an explanation for BLD10/CEP135's role in assembling highly stable MT-based structures, such as motile axonemes and centrioles.
Fundação para a Ciência e Tecnologia grants: (PTDC/BIA-BCM/105602/2008); EMBO Installation Grant; Instituto Gulbenkian de Ciência; EMBO YIP Program; European Research Council grant: ([FP7/2010]/ERC Grant “261344-CentrioleStructNumber.”); Ciência 2007; EMBO, Marie Curie Actions.
