Author(s): Fontagné, Stéphanie ; Silva, Nadia ; Bazin, Didier ; Ramos, Angela ; Aguirre, Peyo ; Surget, Anne ; Abrantes, António ; Kaushik, Sadasivam J. ; Power, Deborah
Date: 2009
Persistent ID: http://hdl.handle.net/10400.1/5428
Origin: Sapientia - Universidade do Algarve
Subject(s): Rainbow trout; Fry; Phosphorous; Calcium; Mineralization; Skeletal development
The effects of dietary levels of phosphorus (P) and calcium(Ca) on skeletal development and mineral deposition in rainbow trout(Oncorhynchus mykiss) fry were studied. Six semi-purified diets were formulated with graded levels of P and Ca. The basal diet A contained only P supplied by casein at 0.5% of drymatter. Other diets B, C,D and Ewere supplementedwith 0.4, 0.8, 1.2 and 1.6% P supplied as a 1:1 mixture of NaH2PO4/KH2PO4 resulting in 0.8, 1.2, 1.6 and 2.2% total P, respectively. These five dietswere supplementedwith 1% Ca supplied as CaCO3whereas another diet F, supplementedwith 0.8% P,was Ca-free. Each dietwas distributed to 3 replicate tanks of 600 swimup fry (initialmean weight: 0.1 g) at a water temperature of 17 °C over a 12-week growth trial. Fish were handfed 6 times a day to visual satiety.
This studywas supported in part by the European Collective research project “Fine Fish” devoted towards understanding the origin of the development of skeletal disorders in larval and juvenile fish (Contract no. COLL-CT-2005-012451). The authors wish to thank F. Terrier, F. Sandres, Y. Hontang and I. Irijalba Claramunt for the care of fish and A. Josserand and M.-J. Borthaire for the technical assistance. They are also very grateful to the radiology department at theHospitalDistrital de Faro, Portugal who assisted in X-ray analysis.
