Biotechnology in aquaculture: polyploidy versus transgenic technology

• Author(s): Ramos, Angela
• Date: 2009
• Persistent ID: http://hdl.handle.net/10400.1/830
• Origin: Sapientia - Universidade do Algarve
• Subject(s): Teses; Aquacultura; Piscicultura; Truta; Biotecnologia; Triplóide
• Document Type: Master thesis
• Language: English
• Advisor(s): Power, Deborah
• Contributor(s): Sapientia

Description

Dissertação mest., Engenharia Biológica, Universidade do Algarve, 2009

Triploidy is the condition in which somatic cells contain three sets of chromosomes which might be a consequence of environmental changes or hybrid stabilization, though in the present work the ploidy induction was done by hyperbare pressure. Diploid and triploid Rainbow trout, Onchorynchus mykiss (Walbaum, 1792) were the species characterized in this thesis. Besides ploidy a modification in the mineral availability of phosphorous, was applied influencing the whole body mineral homeostasis in diploid and triploid trout. The skeletogenesis in triploids animal is different from the diploid, having a general delay, the mineral deficient diet has also an impact on the ossification retreating. It allowed studying the development through triploids, which at the end of this trial they grew less than diploids. Vertebra area is also affected varying acconding to their regions, total number of vertebra differs, and in average triploids have less one vertebra than diploid groups. And there are also histological differences at a muscle level, having triploid Diet A less myotomes in average. Animals present a high capacity in recovering, once they start eating a normal diet in mineral composition, the restore their mineral content in bone structures in some situation an over calcification is observe (Diploid A). In a molecular level, there were any molecular differences observed only a high variability at the OSN levels.