Author(s): Pinto, Patrícia Sofia Gonçalves Carvalho
Date: 2010
Persistent ID: http://hdl.handle.net/10400.5/2469
Origin: Repositório da UTL
Subject(s): Vitis vinifera; Petri disease; Phaeomoniella chamydospora; Phaeoacremonium spp.; pathogenicity; disease control; patogenicidade; meios de luta
Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
Phaeomoniella (Pa.) chlamydospora and Phaeoacremonium spp. are responsible for causing black measles (esca) and Petri disease of mature and young grapevines, respectively. The economic importance of these diseases has increased dramatically worldwide, thus explaining why they have long been the subject of intense research. In this study, the pathogenicity of seven isolates of Pa. chlamydospora to Touriga Nacional vines was confirmed, using two methods of inoculation, spore suspensions and mycelial discs. The inoculation by mycelial discs was significantly more efficient, by the largest size of the necroses produced and the highest percentage of reisolation. There were no differences in virulence among isolates. The efficacy of fungicides in the protection of pruning wounds was evaluated in the varieties Touriga Nacional and Syrah, using carbendazim+flusilazole as reference. Among the parameters evaluated, the percentage of reisolation and/or the detection of the pathogen by nested-PCR were those which proved to be the most reliable. It was observed that the colonization of the vine occurred mainly above and below the 3rd node. For the Syrah grape variety, two treatments significantly reduced the percentage of reisolation of Pa. chlamydospora compared to the control, but they did not differ from the reference fungicide. For Touriga Nacional, only one treatment was comparable to the reference (carbendazim+flusilazol).
