Author(s): Cardoso, Margarida do Nascimento
Date: 2011
Persistent ID: http://hdl.handle.net/10400.5/4114
Origin: Repositório da UTL
Subject(s): black foot; Ilyonectria spp; Cylindrocarpon spp; nested-PCR; multiplex nested-PCR; microbiological isolation
Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
Black foot of grapevine is an important disease caused primarily by fungi of the genus Ilyonectria. These pathogens affect grapevine nurseries and young vineyards, causing the decline and death of plants. In the nursery, the infections are mainly attributed to infected propagation material and soilborne inoculum, thus hampering the selection of healthy plant material. The aim of this study was to detect/identify possible sources of inoculum for Ilyonectria spp. and "Cylindrocarpon" pauciseptatum, also associated with the disease, throughout the different nursery stages. Samples of plant material (rootstock and scion mother plants, grafted-cuttings and rooted graftlings), soil (from mother-fields, nurseries and plots in rotation), callusing-medium (before and after utilization), air chambers and warehouses, water from hydration tanks and tap water, cutting tools and paraffin, were analysed by classical and molecular techniques (nested-PCR and multiplex nested-PCR). Multiplex nested-PCR revealed to be more sensitive and fast than classical technique, allowing the simultaneous detection of I. liriodendri and/or I. macrodidyma and/or "C." pauciseptatum and the identification of unknown inoculum sources for these fungi (eg. callusing-medium and water samples). These studies should be pursued in order to confirm the viability of the inoculum detected.
