Maturação em queijo dos Açores. Determinação de ácidos orgânicos e metabolismo do lactato

• Author(s): Costa, Isadora Barata Braz de Assunção
• Date: 2012
• Persistent ID: http://hdl.handle.net/10400.5/5295
• Origin: Repositório da UTL
• Subject(s): cheese; ripening; organic acids; HPLC; validation; Azores
• Document Type: Master thesis
• Language: Portuguese
• Advisor(s): Martins, António Pedro Louro; Duthoit, Teresa Margarida
• Contributor(s): Repositório da Universidade de Lisboa

Description

Mestrado em Engenharia Alimentar - Processamento de Alimentos - Instituto Superior de Agronomia

The secondary reactions that occur during the ripening of cheese involve complex biochemical changes that affect the final characteristics of the product. Organic acids can be originated from the metabolism of lactate and can significantly influence the development of the sensory properties of the cheese during ripening. This work was carried out to study and develop an HPLC methodology to analyze and quantify organic acids (acetic, butyric, lactic, oxalic and propionic) in cheese. The evolution of the organic acids was then studied during the ripening of three Azorean cheeses. It was concluded that, in general, the organic acids content increased over the cheese ripening and that the organic acids content and profile varied with cheese type; propionic and butyric acids are found to be the most significant in São Jorge cheese. The Cluster Analysis and the Principal Components Analysis showed that the samples were grouped according to manufacturing technology and ripening time.