Publicação

Analysis of NGM STR loci in South Portuguese Population

Detalhes bibliográficos
Resumo:	Allele frequencies and other relevant forensic parameters of 15 loci studied with Applied Biosystems AmpFlSTRs NGM Kit were calculated in a population of individuals residing in the South of Portugal. Blood stain samples were obtained from a total of 350 unrelated individuals involved in paternity testing casework. This new kit has five loci not present in any other kit used in our laboratory (Promega Powerplex 16 System and Applied Biosystems Identifiler Plus): D10S1248, D22S1045, D2S441, D1S1656 and D12S391. The NGM multiplex kit is used in our forensic casework as an auxiliary screening tool to solve deficient casework such as fatherless paternity testing, and to help in paternity investigations with one genetic incompatibility after the routine seventeen loci typing. Furthermore, this five loci included in the European Standard Set are also recommended by the European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling group (EDNAP). These studies are necessary to calculate statistical forensic parameters such as power of discrimination, power of exclusion or minimum allele frequencies. Statistical parameters such as heterozigoty, homozigoty or allele frequencies were determined with Arlequim V3.5 and results compared with the ones reported in previous similar studies. As final remark, is important to remember that this kind of study is highly important for the Forensic Laboratories accreditation achievement
Autores principais:	Ribeiro, Teresa
Outros Autores:	Dario, Paulo; Vital, Nádia; Sanches, Susana; Espinheira, Rosa; Geada, Helena; Costa Santos, Jorge
Assunto:	Genética Forense Estudo Populacional Investigação de Paternidade Forensic Parameters Paternity Investigation Population Study NGM Kit
Ano:	2011
País:	Portugal
Tipo de documento:	documento de conferência
Tipo de acesso:	acesso aberto
Instituição associada:	Instituto Nacional de Medicina Legal e Ciências Forenses, IP
Idioma:	inglês
Origem:	Instituto Nacional de Medicina Legal e Ciências Forenses, IP

Descrição
Resumo:	Allele frequencies and other relevant forensic parameters of 15 loci studied with Applied Biosystems AmpFlSTRs NGM Kit were calculated in a population of individuals residing in the South of Portugal. Blood stain samples were obtained from a total of 350 unrelated individuals involved in paternity testing casework. This new kit has five loci not present in any other kit used in our laboratory (Promega Powerplex 16 System and Applied Biosystems Identifiler Plus): D10S1248, D22S1045, D2S441, D1S1656 and D12S391. The NGM multiplex kit is used in our forensic casework as an auxiliary screening tool to solve deficient casework such as fatherless paternity testing, and to help in paternity investigations with one genetic incompatibility after the routine seventeen loci typing. Furthermore, this five loci included in the European Standard Set are also recommended by the European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling group (EDNAP). These studies are necessary to calculate statistical forensic parameters such as power of discrimination, power of exclusion or minimum allele frequencies. Statistical parameters such as heterozigoty, homozigoty or allele frequencies were determined with Arlequim V3.5 and results compared with the ones reported in previous similar studies. As final remark, is important to remember that this kind of study is highly important for the Forensic Laboratories accreditation achievement