Expression-based and co-localization detection of arabinogalactan protein 6 and arabinogalactan protein 11 interactors in Arabidopsis pollen and pollen tubes

• Author(s): Costa, Mário ; Nobre, Margarida Sofia ; Becker, Jörg D ; Masiero, Simona ; Amorim, Maria Isabel ; Pereira, Luís Gustavo ; Coimbra, Sílvia
• Date: 2013
• Persistent ID: http://hdl.handle.net/10400.7/691
• Origin: ARCA - Access to Research and Communication Annals
• Subject(s): Arabidopsis Proteins; Gene Expression Regulation, Plant; Mucoproteins; Plant Proteins; Pollen; Pollen Tube
• Document Type: Journal article
• Language: English
• Contributor(s): ARCA

Description

Arabinogalactan proteins (AGPs) are cell wall proteoglycans that have been shown to be important for pollen development. An Arabidopsis double null mutant for two pollen-specific AGPs (agp6 agp11) showed reduced pollen tube growth and compromised response to germination cues in vivo. A microarray experiment was performed on agp6 agp11 pollen tubes to search for genetic interactions in the context of pollen tube growth. A yeast two-hybrid experiment for AGP6 and AGP11 was also designed.