Autor(es):
Rodrigues, Cristiana ; Laranjeira, Paula ; Pinho, A. C. O. ; Silva, Isabel ; Silva, Sandra ; Coucelo, Margarida ; Oliveira, Ana Catarina ; Simões, Ana Teresa ; Damásio, Inês ; Silva, Helena Matos ; Urbano, Mafalda ; Sarmento Ribeiro, Ana Bela ; Geraldes, Catarina ; Domingues, Maria Rosario ; Almeida, Julia ; Criado, Ignacio ; Orfao, Alberto ; Paiva, Artur
Data: 2024
Identificador Persistente: http://hdl.handle.net/10400.26/50636
Origem: Instituto Politécnico de Coimbra
Assunto(s): CD20+ T cells; chronic lymphocytic leukemia; monoclonal B lymphocytosis; immunoglobulin heavy chain variable region; T cell polarization; leucemia linfocítica crónica de células B; cadeias pesadas de imunoglobulinas
Descrição
Introduction: In monoclonal B cell lymphocytosis (MBL) and chronic lymphocytic leukemia (CLL), the expansion of malignant B cells disrupts the normal homeostasis and interactions between B cells and T cells, leading to immune dysregulation. CD20+ T cells are a subpopulation of T cells that appear to be involved in autoimmune diseases and cancer. Methods: Here, we quantified and phenotypically characterized CD20+ T cells from MBL subjects and CLL patients using flow cytometry and correlated our findings with the B-cell receptor mutational status and other features of the disease. Results and discussion: CD20+ T cells were more represented within the CD8+T cell compartment and they showed a predominant memory Tc1 phenotype. CD20+ T cells were less represented in MBL and CLL patients vs healthy controls, particularly among those with unmutated IGVH gene. The expansion of malignant B cells was accompanied by phenotypic and functional changes in CD20+ T cells, including an increase in follicular helper CD4+ CD20+ T cells and CD20+ Tc1 cells, in addition to the expansion of the TCR Vb 5.1 in CD4+ CD20+T cells in CLL.
