Author(s): Semião-Santos, Saul ; Barral Veloso, Laura ; Paes de Andrade, Paulo ; Almeida de Melo, Márcia ; Martins, Luís ; Marinho, Artur ; Afonso de Almeida, José ; Campino, Lénea ; Harith, Abdallah
Date: 2015
Persistent ID: http://hdl.handle.net/10174/12571
Origin: Repositório Científico da Universidade de Évora
Subject(s): Leishmania; DAT; b-mercaptoethanolmodified; antigen
We compared the performance of a locally produced b-mercaptoethanol-modified promastigote antigen (b-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for b-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100% were estimated for b-ME-Ag in comparison with 96.6% and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s k50.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of b-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.
