Author(s): Branco, P ; Arantes, S ; Candeias, A ; Caldeira, A T ; González-Pérez, M
Date: 2019
Persistent ID: http://hdl.handle.net/10174/24024
Origin: Repositório Científico da Universidade de Évora
Subject(s): Fluorescence In Situ Hybridization; Cultural Heritage; Rhodotorula sp.; RNA-FISH probes
To distinguish Rhodotorula sp., from other microorganisms that produce the same type of alterations on CH materials, proper identification methods must be applied. RNA-fluorescence and in situ hybridization (RNA-FISH) has the potential to specifically identify the target microorganism of interest in complex microbial communities (it is based on hybridization of fluorescently-labeled oligonucleotide probes targeting to specific regions of the ribosomal RNA). Thus, the aim of this study was to design a novel genus specific RNAFISH probe against Rhodotorula sp., and to evaluate its specificity and performance both in silico and experimentally. This will contribute for facilitating Rhodotorula sp., identification in degraded CH materials by RNA-FISH.
This work was co-financed by FCT through PTDC/BBB-IMG/0046/2014 project and SFRH/BPD/100754/2014 grant and by ALT20-03-0246-FEDER-000004-ALENTEJO 2020 project.
