Stereological Estimation of Mean Nuclear Volume as a Prognostic Factor in Canine Mast Cell Tumors

• Autor(es): Casanova, M ; Branco, S ; Veiga, I ; Faísca, P
• Data: 2021
• Identificador Persistente: http://hdl.handle.net/10174/29246
• Origem: Repositório Científico da Universidade de Évora
• Assunto(s): mast cell tumours; canine; mean nuclear volume
• Tipo de Documento: Artigo científico
• Idioma: Português

Descrição

Cutaneous mast cell tumour (MCT) Patnaik and Kiupel grading schemes rely on qualitative and semiquantitative features susceptible to interobserver variability. Stereological estimation of volume-weighted mean nuclear volume (MNV) provides information about both size and variability of nuclear size, which has been proven to have a prognostic value in other solid tumours. The objective was to compare MNV with MCT grade and biological behaviour. Fifty-six MCTs were graded according to Patnaik and Kiupel by consensus of three experienced pathologists.Clinical history of dogs treated with surgical excision alone was collected with a minimum follow-up period of 1 year (n=31).MNV was estimated using the point-intercept method on vertical sections in 10 microscopical fields, with an approximately constant distance proportional to overall sectional area. Animals were divided according to outcome: group 1, no recurrence; group 2, local recurrence, lymph node or distant metastasis. The present study suggests that estimation of MNV on routine histological sections may objectively improve the detection of more aggressive MCTs.