Author(s): Eustáquio, R. ; Ramalho, J.P.P. ; Caldeira, A.T. ; Pereira, A.
Date: 2023
Persistent ID: http://hdl.handle.net/10174/33069
Origin: Repositório Científico da Universidade de Évora
Subject(s): coumarin; 4-styrylcoumarin derivatives; fluorescent labels; biomolecules labeling; large Stokes shifts
Important scientific areas, such as cellular biology, medicine, pharmacy, and environmental sciences, are dependent on very sensitive analytical techniques to track and detect biomolecules. In this work, we develop a simple, low-cost and effective synthetic strategy to produce new red-shifted 4-styrylcoumarin derivatives as promising inexpensive fluorescent labels for biomolecules. The extension of the delocalized -electron system results in bathochromic shifts in these new coumarin derivatives, which also present large Stokes shifts. In addition, density functional theory and timedependent density functional theory calculations helped to rationalize the photophysical properties observed by the experimental results.
The study was performed under the framework of THE SCREAM Project- “Touchstone for Heritage Endangered by Salt Crystallization, a Research Enterprise on the Art of Munch” (ref. FCT-ALT20-03-0145-FEDER-031577) financed by the Portuguese Foundation for Science and Technology (FCT) through National and European Funds, PROBIOMA project (0483_PROBIOMA_ 5_E, EP), co-financed by the FEDER, through the programme INTERREG VA España— Portugal (POCTEP) and the City University of Macau endowment to the Sustainable Heritage Chair.
