The potential of enzymatic hydrolysis with HS-SPME-GC×GC-ToFMS for the study of volatile signature of grapes

• Author(s): Fonseca, Daniela ; Martins, Nuno ; Garcia, Raquel ; Cabrita, Maria João
• Date: 2025
• Persistent ID: http://hdl.handle.net/10174/39076
• Origin: Repositório Científico da Universidade de Évora
• Subject(s): Aroma composition; HS-SPME-GC×GC-ToFMS; AR2000 enzyme; Clones
• Document Type: Journal article
• Language: English

Description

This works focuses on developing an optimized methodology for analyzing free and bound volatile compounds in grapes using enzymatic hydrolysis with AR2000 enzyme and HS-SPME-GC × GC-ToFMS. To achieve this purpose, a previous optimization step of enzymatic hydrolysis was implemented and optimal conditions for enzymatic hydrolysis were established using 50 mg of AR2000 enzyme at 35 °C for 24 h, while the best extraction conditions were found to be 40 min at 60 °C. Applying this methodology to clones of Moreto grapes from harvest of 2022 and 2023, it was possible to identify and semi-quantify sixty volatile compounds, with more compounds identifiable in 2022 than in 2023. The results also showed the influence of crop year and genetic intravariability.