Author(s): Conte, Fernanda Lopes ; Pereira, Ana Catarina ; Brites, Gonçalo ; Ferreira, Isabel ; Silva, Ana Cristina ; Sebastião, Ana Isabel ; Matos, Patrícia ; Pereira, Cláudia ; Batista, Maria Teresa ; Sforcin, José Maurício ; Cruz, Maria Teresa
Date: 2022
Persistent ID: https://hdl.handle.net/10316/100510
Origin: Estudo Geral - Universidade de Coimbra
Subject(s): Anti-inflammatory action; Antiallergic action; Antioxidant activity; Monocyte; Propolis
Propolis is a bee product used since ancient times due to its diverse biological applications. We aimed to investigate the antioxidant and anti-inflammatory action of Brazilian propolis in THP-1 cells stimulated with 1‐fluoro‐2,4‐dinitrobenzene (DNFB) or lipopolysaccharide (LPS). Methods: Cell viability was assessed by resazurin assay, antioxidant activity was evaluated by DPPH assay, superoxide dismutase (SOD) 1 and 2 activity was evaluated by colorimetric assay. CD86 expression was determined by flow cytometry. IL-1β and HMOX-1 were analysed by Western blot. Results: Propolis did not affect cell viability and exhibited a potent antioxidant activity. Propolis alone induced SOD 1 activity. LPS stimulated SOD2, concomitantly or not with propolis. In the presence of LPS, propolis induced a higher HMOX-1 expression. Propolis inhibited CD86 expression stimulated by DNFB. LPS induced pro-IL-1β expression and propolis did not affect its action. Conclusion: Propolis exhibited an antioxidant action in a cell-free system and in a cell based-model. Propolis exerted an anti-inflammatory/antiallergic action, probably due to its antioxidant activity.
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior/Programa Institucional de Internacionalização (CAPES/PRINT) under grant number: 88887.467268/2019–00.
