Generation and characterization of a human iPS cell line from a patient-related control to study disease mechanisms associated with DAND5 p.R152H alteration

• Autor(es): Pars, Selin ; Cristo, Fernando ; Inácio, José M. ; Rosas, Graça ; Carreira, Isabel Marques ; Melo, Joana Barbosa ; Mendes, Patrícia ; Martins, Duarte Saraiva ; Almeida, Luís Pereira de ; Maio, José ; Anjos, Rui ; Belo, José A.
• Data: 2018
• Identificador Persistente: https://hdl.handle.net/10316/108062
• Origem: Estudo Geral - Universidade de Coimbra
• Projeto/bolsa: info:eu-repo/grantAgreement/other/Multi/04462/other
• Assunto(s): Cell Line; Humans; Male; Cellular Reprogramming Techniques; Heart Diseases; Induced Pluripotent Stem Cells; Intercellular Signaling Peptides and Proteins
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited as a control iPSC line to better understand the mechanisms involved in DAND5-associated cardiac disease.