Autor(es):
González-Ceballos, Lara ; Carlos Guirado-Moreno, Jose ; Utzeri, Gianluca ; Miguel García, José ; Fernández-Muiño, Miguel A. ; Osés, Sandra M. ; Teresa Sancho, M. ; Arnaiz, Ana ; Valente, Artur J. M. ; Vallejos, Saul
Data: 2023
Identificador Persistente: https://hdl.handle.net/10316/113737
Origem: Estudo Geral - Universidade de Coimbra
Assunto(s): Catechol; 1,2-Dihydroxybenzene; Phenylboronic acid Glucose oxidase; Catalase; Honey; Glucose Oxidase; Catalase; Polyphenols; Hydrogen Peroxide; Polymers; Renal Dialysis; Glucose; Honey
Descrição
Glucose oxidase (GOX) and catalase (CAT) regulate the amount of H2O2 in honey, by generating or consuming it, so they are related to the antibacterial and antioxidant activity of honey. However, their activities are hardly analysed, since the process requires a previous dialysis that is non-selective, very time-consuming (>24 h), eco-unfriendly (>6L of buffer) and expensive. This research shows the design and performance of a material that selectively removes the actual interferents. The film-shaped-polymer is immersed for 90́ within a honey solution (12.5 mL of buffer), where it interacts exclusively with 1,2-dihydroxybenzenes, which we proved to be the real interferents (the material contains motifs derived from phenylboronic acid to interact with 1,2-diols). Polymeric chains favour condensation to occur exclusively with 1,2-dihydroxybenzenes, excluding monosaccharides. The interferents' removal using our designed polymer is selective, low cost (1.42€ per test), rapid and eco-friendly (saves 6L of buffer and 20.5 h of experimental workout per sample).
PID2020-113264RBI00; AEI/10.13039/501100011033; MCIN/AEI/10.13039/501100011033
