Autor(es): Gonçalves, Paula P. ; Meireles, Sandra M. ; Neves, Paulo ; Vale, M. Graça P.
Data: 1999
Identificador Persistente: https://hdl.handle.net/10316/5465
Origem: Estudo Geral - Universidade de Coimbra
Assunto(s): Cations transport; Ca2+/H+ antiport; Synaptic vesicle; Brain cortex
As we previously reported, synaptic vesicles isolated from sheep brain cortex contain a Ca2+/H+ antiport that permits Ca2+ accumulation inside the vesicles (~5 nmol/mg protein) at expenses of the pH gradient generated by the H+-pumping ATPase. We observed that the system associates Ca2+ influx to H+ release and operates with low affinity for Ca2+. In the present work, we found that Ca2+/H+ antiport mediates exchange of protons with other cations such as Zn2+ and Cd2+, suggesting that these cations and Ca2+ share the same transporter molecules to enter the intravesicular space. Zn2+ and Cd2+ induce H+ release in a concentration-dependent manner (fluorimetrically evaluated) and they inhibit the antiport-mediated Ca2+ uptake by the vesicles (isotopically measured). In contrast, large cations such as Ba2+ and Cs+ do not alter Ca2+ influx and they are unable to induce proton release from the vesicles. With respect to Sr2+, which has an intermediary size relatively to the other groups of cations, we found that it does not induce H+ liberation from the vesicles, but it has a concentration-dependent inhibitory effect on the Ca2+-induced H+ release and Ca2+ uptake by the vesicles. These results indicate that the cation selectivity of the synaptic vesicles Ca2+/H+ antiport is essentially determined by the size of the cation transported into the vesicles.
http://www.sciencedirect.com/science/article/B6T07-3W9CWTS-9/1/5dba905676aadcbf1c390548cc657219
