Author(s): Gusmão, Michélia Antônia do Nascimento ; Emídio, Nayara Braga ; Marconato, Danielle Gomes ; Farani, Priscila Silva Grijó ; Maniezzi, Luiz Felipe ; Coelho, Paulo Marcos Zech ; Oliveira, Rodrigo Correa ; Vasconcelos, Eveline Gomes ; Faria-Pinto, Priscila de
Date: 2020
Origin: Oasisbr
Subject(s): -; ATP diphosphohydrolase; IgE; Potato apyrase
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Introduction: High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins. Methods: Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity. Results: Most of the schistosomiasis patient’s (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production. Conclusions: Potato apyrase and SmATPDases have IgE-binding epitopes.
