Sele????o de bact??rias endof??ticas amaz??nicas produtoras de lipopept??deos bioativos

• Author(s): Mesquita, Adriana Spirotto Stein
• Date: 2016
• Origin: Oasisbr
• Subject(s): Lipopept??deos; Bact??rias endof??ticas; Espectrometria de massas; Lipopeptides; Endophytic bacteria; Mass spectrometry; CI??NCIAS EXATAS E DA TERRA: QU??MICA
• Document Type: Master thesis
• Language: Portuguese

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CAPES - Coordena????o de Aperfei??oamento de Pessoal de N??vel Superior

The endophytic microrganisms, particularly bacteria and fungi live in the plants, including thein roots apparently cause no harm. Bacteria and fungi are potentially useful in agriculture and industry, particularly in the food and pharmaceutical industries, as they produce different chemical classes and can participate in various industrial biotechnological processes. Among the compounds produced by bacteria, lipopeptides belong to a class that has attracted great interest for its antifungal, antibiotic and surfactant among others. In this stud 50 strains were selected of Amazonian endophytic bacteria for antimicrobial evoluction and the production of lipopeptides by ESI-ITMS2. Of these, 25 strains produced lipopeptides, six plant isolated from Gustavia genus, nine Macrophyte, five Duguetia, one Pothomorpha, two Rollina and one Bryophyllum. The fragmentation analysis by ESI-MS2 in negative mode identified eight extracts the lipopeptide surfactin homologs of C11 - C18, including C11, C17 and C18 not been reported in the literature. In biological assays paired cultures, 48 strains showed mycoparasitism antimicrobial activity against Gram-negative bacteria P. aeruginosa and E. coli, 27 strains against Gram-positive bacteria S. aureus, E. faecalis, 29 strains against and 28 against C. albicans, also presenting competition activity against E. faecalis 21 strains and C. albicans strains against 23 S. aureus strains and 2 against P.aeruginosas and E. coli. No strain with antibiotic activity observed. In assessing the bioactivity of lipopeptide extracts, 13 of the extracts strains showed antibiosis against S. aureus, P. aeruginosa and C.albicans.

Os microrganismos endof??ticos, especialmente bact??rias e fungos, s??o assim denominados por viverem no interior das plantas, habitando inclusive suas ra??zes, sem lhes causar aparentemente nenhum dano. As bact??rias e os fungos s??o potencialmente ??teis na agricultura e na ind??stria, particularmente na aliment??cia e farmac??utica, pois produzem in??meras classes de subst??ncias e podem participar de processos biotecnol??gicos industriais diversos. Entre as subst??ncias produzidas por bact??rias, os lipopept??deos s??o uma classe que tem atra??do grande interesse por suas atividades antif??ngica, antibi??tica e surfactante entre outras. No presente trabalho foram selecionadas 50 linhagens de bact??rias endof??ticas amaz??nicas para serem avaliadas por ensaios antimicrobianos e quanto ?? produ????o de lipopept??deos por ESI-ITMS2. Destas, 25 linhagens produziram lipopept??deos, sendo seis isoladas de planta do g??nero Gustavia, nove de Macrofita, cinco de Duguetia, uma de Pothomorpha, duas de Rollina e uma de Bryophyllum. As an??lises de fragmenta????o por ESI-MS2 no modo negativo dos extratos lipopept??dicos identificaram oito hom??logos da surfactina de C11 - C18, entre os quais o C11, C17 e C18 ainda n??o foram relatados na literatura. Em ensaios biol??gicos de culturas pareadas, 48 linhagens apresentaram atividade antimicrobiana de microparasitismo contra as bact??rias Gram-negativas P. aeruginosa e E. coli, 27 linhagens contra as bact??rias Gram-positiva S. aureus, 29 linhagens contra E. faecalis e 28 contra C. albicans, apresentando tamb??m atividade de competi????o frente a 21 linhagens contra E. faecalis e C. albicans, 23 linhagens contra S. aureus e 2 linhagens contra P.aeruginosa e E. coli. N??o foi observada nenhuma linhagem com atividade antibi??tica. Na avalia????o da bioatividade dos extratos lipopept??dicos, 13 extratos destas linhagens apresentaram antibiose frente a S. aureus, P. aeruginosa e C. albicans.