Author(s): Cazarin, Cinthia Baú Betim, 1979- ; Maróstica Junior, Mário Roberto, 1980-
Date: 2019
Persistent ID: https://hdl.handle.net/20.500.12733/1649085
Origin: Oasisbr
Subject(s): Uva; Compostos fenólicos; Apoptose; Apoptosis; Grapes; Phenolic compounds; Colon; Inflammation; Caspase; Artigo original
Agradecimentos: Fellowships from National Council for Scientific and Technological Development (CNPq), financial support from CNPq (grant numbers 552440/2011-6, 309227/2013-5, 458664/2014-6, 301108/2016-1, 403328/2016-0), São Paulo Research Foundation (FAPESP grant number #2015/50333-1), and Coordination for the Improvement of Higher Education Personnel (CAPES finance code 001) have supported this work
Abstract: Ulcerative colitis (UC) is a chronic inflammatory bowel disease that reduces glutathione (GSH) levels. We aimed to investigate the effect of dietary supplementation with grape peel powder (GPP) on the GSH system in a rat model of colitis induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS) and to identify the role of grape bioactive fractions, namely soluble phenolics (EP), fiber-bound polyphenols (NEP-F), and dietary fiber (F). Colitis reduced colonic GSH levels and glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) activities. NEP-F completely restored GR and GPx, while all diets restored GST activities. Colitis increased the mRNA expression of both subunits of glutamate-cysteine ligase and induced apoptosis in the colon. The reestablishment of GSH levels promoted by GPP was associated with the recycling of oxidized glutathione by increased GR activity instead of GSH synthesis. NEP-F and F fractions were responsible for the protective effect of GPP against apoptosis
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
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