Author(s):
Shimojo, Andréa Arruda Martins, 1971- ; Brissac, Isabela Cambraia de Souza, 1992- ; Pina, Lucas Martins, 1995- ; Lambert, Carlos Salles ; Santana, Maria Helena Andrade, 1951-
Date: 2015
Persistent ID: https://hdl.handle.net/20.500.12733/1655196
Origin: Oasisbr
Subject(s): Ácido hialurônico; Hyaluronic acid; Ethanol; Sterilization; Ultraviolet radiation; Auto-crosslinked hyaluronic acid; Ethanol treatment; Artigo de pesquisa; Etanol; Esterilização; Radiação ultravioleta; Plasma radiation; Steam
Description
Agradecimentos: The authors acknowledge the financial support from the National Council of Technological and Scientific Development (CNPq, Brazil). They would also like to thank Prof. Dr. Edvaldo Sabadini of the Chemical Institute (University of Campinas) for the use of the rheometer
Abstract: This work evaluated the effects of UV irradiation, plasma radiation, steam and 70% ethanol treatments on the sterilization and integrity of auto-crosslinked hyaluronic acid (HA-ACP) scaffolds structured in microparticles and sponges aiming in vivo applications for regenerative medicine of bone tissue. The integrity of the microparticles was characterized by rheological behavior, while for the sponges, it was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The effectiveness of the sterilization treatment was verified by the number of microorganism colonies in the samples after the treatments. In conclusion, plasma radiation was the best treatment for the sponges, while steam sterilization in the autoclave at 126°C (1.5 kgf/cm2) for 5 min was the best treatment for the microparticles
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
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