Author(s):
Dourado, Mauricio da Rocha, 1989- ; Mofatto, Luciana Souto, 1979- ; Bastos, Débora Campanella, 1981- ; Messetti, Ana Camila Pereira, 1980- ; Graner, Edgard, 1968- ; Della Coletta, Ricardo, 1972-
Date: 2019
Persistent ID: https://hdl.handle.net/20.500.12733/1657294
Origin: Oasisbr
Subject(s): Câncer; Microambiente tumoral; Fibroblastos; Migração; Vesículas extracelulares; Extracellular vesicles; Fibroblasts; Cancer; Emigration and immigration; Microambiente tumoral; Oral cancer-associated fibroblasts; Artigo de pesquisa
Description
Agradecimentos: MRD was supported by grants from the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior - CAPES, Brasilia, Brazil [Finance Code 001] and Medical Research Center, University of Oulu, Finland. JK was supported by a grant from the Finnish Cultural Foundation [Grant number 00130432]. RDC was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior - CAPES, Brasilia, Brazil [AUXPE-PVES-570/2013]. This study was supported by the grants from the Finnish Cancer Society, The Sigrid Juselius Foundation, and the Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq, Brasíia, Brazil [302964/2015-0]
Abstract: As one of the most abundant constituents of the tumour microenvironment (TME), cancer-associated fibroblasts (CAF) display critical roles during tumour progression and metastasis. Multiple classes of molecules including growth factors, cytokines, proteases and extracellular matrix proteins, are produced by CAF to act as mediators of the stroma-tumour interactions. One of the main channels for this communication is associated with extracellular vesicles (EV), which are secreted particles loaded with protein and genetic information. In this study, we evaluated the effects of EV derived from CAF primary human cell lines (n = 5) on proliferation, survival, migration, and invasion of oral squamous cell carcinoma (OSCC) cells. As controls, EV from human primary-established normal oral fibroblasts (NOF, n = 5) were used. Our in vitro assays showed that CAF-EV significantly induces migration and invasion of OSCC cells and promote a disseminated pattern of HSC-3 cell invasion in the 3D organotypic assay. Furthermore, gene expression analysis of EV-treated cancer cells revealed changes in the pathways associated with tumour metabolism and up-regulation of tumour invasion genes. Our findings suggest a significant role of CAF-EV in promoting the migration and invasion of OSCC cells, which are related to the activation of cancer-related pathways
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPES
Fechado
