Author(s):
Teixeira, Paulo José Pereira Lima, 1986- ; Thomazella, Daniela Paula de Toledo, 1984- ; Reis Junior, Osvaldo, 1986- ; Prado, Paula Favoretti Vital do, 1991- ; Rio, Maria Carolina Scatolin do, 1977- ; José, Juliana ; Costa, Gustavo Gilson Lacerda ; Negri, Víctor Augusti, 1986- ; Pereira, Gonçalo Amarante Guimarães, 1964-
Date: 2014
Persistent ID: https://hdl.handle.net/20.500.12733/127
Origin: Oasisbr
Subject(s): Moniliophthora perniciosa; Senescência foliar; Moniliophthora perniciosa; Leaf senescence; Artigo original
Description
Agradecimentos: We thank Halley Caixeta de Oliveira and Li Yang for valuable suggestions and critical reading of the article, Marc Lohse for his assistance with the MapMan annotation, Ramon Vidal for assistance with the bioinformatics, and Ricardo Silverio Machado for helping with the photosynthesis measurements. We thank the University of North Carolina High Throughput Sequencing Facility team for intense support with RNA-seq. This work was supported by the Sao Paulo Research Foundation (FAPESP, Grants 2009/51018-1, 2009/50119-9, 2006/59843-3, 2008/54527, and 2012/09136-0)
Abstract: Witches' broom disease (WBD), caused by the hemibiotrophic fungus Moniliophthora perniciosa, is one of the most devastating diseases of Theobroma cacao, the chocolate tree. In contrast to other hemibiotrophic interactions, the WBD biotrophic stage lasts for months and is responsible for the most distinctive symptoms of the disease, which comprise drastic morphological changes in the infected shoots. Here, we used the dual RNA-seq approach to simultaneously assess the transcriptomes of cacao and M. perniciosa during their peculiar biotrophic interaction. Infection with M. perniciosa triggers massive metabolic reprogramming in the diseased tissues. Although apparently vigorous, the infected shoots are energetically expensive structures characterized by the induction of ineffective defense responses and by a clear carbon deprivation signature. Remarkably, the infection culminates in the establishment of a senescence process in the host, which signals the end of the WBD biotrophic stage. We analyzed the pathogen's transcriptome in unprecedented detail and thereby characterized the fungal nutritional and infection strategies during WBD and identified putative virulence effectors. Interestingly, M. perniciosa biotrophic mycelia develop as long-termparasites that orchestrate changes in plant metabolism to increase the availability of soluble nutrients before plant death. Collectively, our results provide unique insight into an intriguing tropical disease and advance our understanding of the development of (hemi) biotrophic plant-pathogen interactions
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
Fechado
