Author(s):
Laurito, Tiago Lüders, 1981- ; França, Flávia Thomé ; Pelegati, Vitor Bianchin, 1982- ; Baratti, Mariana Ozello, 1980- ; Carvalho, Hernandes Faustino de, 1965- ; César, Carlos Lenz, 1955- ; Moraes, Aparecida Machado de, 1958- ; Cintra, Maria Letícia, 1951- ; Teixeira, Fernanda
Date: 2021
Persistent ID: https://hdl.handle.net/20.500.12733/2796
Origin: Oasisbr
Subject(s): Fibra de colágeno; Carcinoma de célula de Merkel; Microscopia óptica não linear; Geração de segundo harmônico; Collagen fiber; Carcinoma, Merkel cell; Nonlinear optical microscopy; Second harmonic generation; Textural analysis; Artigo de pesquisa
Description
Agradecimentos: The equipment used for the development of this work was purchased with the help of Fapesp (the São Paulo Research Foundation; 08/57906-3) and CNPq (the National Council for Scientific and Technological Development; 573913/2008-0). We reviewed the content of the manuscript, followed by Ms Diane Ellis, B.A. in education. Biostatistician Cleide Aparecida Moreira Silva, Research Committee, Biostatistics Division, Medical Sciences School, Unicamp, provided statistical consultation
Abstract: Solid tumors typically contain high levels of fibrillar collagen. The increased stromal collagen deposition usually promotes cancer progression since biochemical and biophysical cues from tumor-associated collagen fibers stimulate neoplastic cells. Few studies have investigated the relationship between Merkel cell carcinoma (MCC) and the extracellular matrix (ECM), but there are no works evaluating collagen. This is an observational, analytical, retrospective study including 11 patients with MCC. Primary tumor-stained sections were evaluated by second harmonic generation microscopy and texture analysis. Peritumoral texture features (area fraction, mean gray value, entropy, and contrast) showed much lower values than normal skin (P < .0001) revealing extensively altered structure of peritumoral collagen fibers. These differences were not significant between tumors with unfavorable and favorable known prognostic factors. Profound changes in collagen fibers present in the stroma accompanying primary MCC may contribute to the aggressive behavior of this tumor. Our results indicate that whatever MCC histological subtype, size or anatomical location, MCC promotes the same type of ECM for its development. As an outlook, therapies using ECM macromolecules or fibroblasts (the architects of ECM remodeling) as target could be useful in the treatment of MCC
FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP
CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQ
Aberto
