Autor(es):
Santos, Priscila Laíse dos ; Araujo, Adriano Antunes de Souza ; Quintans, Jullyana de Souza Siqueira ; Brito, Renan Guedes de ; Serafini, Mairim Russo ; Menezes, Paula dos Passos ; Santos, Márcio Roberto Viana dos ; Alves, Péricles Barreto ; Lucca Junior, Waldecy de ; Blank, Arie Fitzgerald ; La Rocca, Viviana ; Almeida, Reinaldo Nobrega de ; Quintans Júnior, Lucindo José
Data: 2018
Origem: Oasisbr
Assunto(s): Cymbopogon winterianus; Essential oils; β-cyclodextrin; Óleos essenciais; β-ciclodextrina
Descrição
This study aimed to evaluate the orofacial antinociceptive effect of the Cymbopogon winterianus essential oil (LEO) complexed in B-cyclodextrin (LEO-CD) and to assess the possible involvement of the central nervous system (CNS). The LEO was extracted, chromatographed, and complexed in B-cyclodextrin. The complex was characterized by differential scanning calorimetry (DSC) and thermogravimetry derivative (TG/DTG). Male Swiss mice (2-3 months) were treated with LEO-CD (50–200 mg/kg, p.o.), vehicle (distilled water, p.o.), or standard drug (i.p.) and subjected to the orofacial nociception formalin-, capsaicin-, and glutamateinduced. After the formalin test, the animals were perfused and the brains subjected to immunofluorescence for Fos. The rota-rod test (7 rpm/min) was carried out. Geraniol (37.57%) was the main compound of LEO. DSC and TG/DTG proved the complexation. The orofacial nociceptive behavior was significantly (p < 0.05) reduced. The number of Fos-positive cells was significantly changed in the dorsal raphe nucleus (p < 0.01), locus coeruleus (p < 0.001), trigeminal nucleus (p < 0.05), and trigeminal thalamic tract (p < 0.05). LEO-CD did not cause changes in motor coordination in the rota-rod test. Thus, our results suggested that LEO-CD has an orofacial antinociceptive profile, probably mediated by the activation of the CNS without changing the motor coordination.
