Author(s): Lucia, Ceres Mattos Della ; Silva, Elizangela Rodrigues da ; Ribeiro, Sônia Machado Rocha ; Pinheiro-Sant'Ana, Helena Maria ; Brandão, Sebastião César Cardoso
Date: 2018
Origin: Oasisbr
Subject(s): Tetrahydrofolate; 5-methyltetrahydrofolate; 5-methyltetrahydrofolate
We optimized a method for the determination of three main folate isoforms in leafy vegetables. Analysis was carried out by HPLC, using gradient of elution and fluorescence detection. Limits of quantification were 2 ng/mL; 2 ng/mL and 28 ng/mL for tetrahydrofolate (THF), 5-methyltetrahydrofolate (5-MTHF) and 5-formyltetrahydrofolate (5-FTHF), respectively. Recovery rates of THF, 5-MTHF and 5-FTHF varied from 87.86 to 100.64%; 88.12-94.22% and 89.82-106.69%, respectively. Test of repeatability showed relative standard deviation for peak areas lower than 10%. Tests for linearity showed high coefficients of determination. The results showed advances in folates extraction and analysis in raw and cooked leafy vegetables.
