A practical and reliable procedure for in vitro induction of tetraploid tomato

• Autor(es): Praça, Milene Miranda ; Carvalho, Carlos Roberto de ; Clarindo, Wellington Ronildo
• Data: 2018
• Origem: Oasisbr
• Assunto(s): Colchicine; Chromosome; Flow cytometry; Solanum lycopersicum
• Tipo de Documento: Artigo científico
• Idioma: Inglês

Descrição

A relatively rapid, practical and reliable procedure for in vitro production of tetraploid tomato using colchicine is described. Using this procedure 11.11% generation of tetraploids was obtained after exposure of seedling shoot meristem explants with 8 mM colchicine for 96 h. Confirmation of tetraploid production (2n = 48), was determined by flow cytometry and verified by cytogenetic analysis of root tip preparations. The results indicate that the procedure is adequate for induction and screening of tetraploid tomato plantlets.