Author(s):
Nunes, M?rcio Roberto Teixeira ; Palacios, Gustavo ; Nunes, Keley N. B ; Casseb, Samir Mansour M ; Martins, L?via Caricio ; Quaresma, Juarez A. S ; Savji, Nazir ; Lipkin, W. Ian ; Vasconcelos, Pedro Fernando da Costa
Date: 2017
Origin: Oasisbr
Subject(s): V?rus da Febre Amarela; Febre Amarela / transmiss?o; Febre Amarela / epidemiologia; Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos; V?rus da Febre Amarela / isolamento & purifica??o; Arbovirus / isolamento & purifica??o; Sa?de P?blica; Flaviviridae
Description
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Department of Pathology. Bel?m, PA, Brazil.
Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA.
Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Department of Pathology. Bel?m, PA, Brazil.
Yellow fever virus (YFV), a member of the family Flaviviridae, genus Flavivirus is endemic to tropical areas of Africa and South America and is among the arboviruses that pose a threat to public health. Recent outbreaks in Brazil, Bolivia, and Paraguay and the observation that vectors capable of transmitting YFV are presenting in urban areas underscore the urgency of improving surveillance and diagnostic methods. Two novel methods (RT-hemi-nested-PCR and SYBR(?) Green qRT-PCR) for efficient detection of YFV strains circulating in South America have been developed. The methods were validated using samples obtained from golden hamsters infected experimentally with wild-type YFV strains as well as human serum and tissue samples with acute disease.
