Autor(es):
Le?o, Sylvia Cardoso ; Viana Niero, Cristina ; Matsumoto, Cristianne Kayoko ; Lima, Karla Valeria Batista ; Lopes, Maria Luiza ; Palaci, Mois?s ; Hadad, David Jamil ; Vinhas, Solange ; Duarte, Rafael Silva ; Louren?o, Maria Cristina Silva ; Kipnis, Andr? ; Neves, Zilah C?ndida das ; Gabardo, Betina Mendez Alc?ntara ; Ribeiro, Marta Os?rio ; Baethgen, Ludmila ; Assis, Denise Brand?o de ; Madalosso, Geraldine ; Chimara, Erica ; Dalcolmo, Margareth Pretti
Data: 2017
Origem: Oasisbr
Assunto(s): Infec??es por Mycobacterium / epidemiologia; Mycobacterium / classifica??o; Infec??o Hospitalar; Impress?es Digitais de DNA; Infec??o da Ferida Operat?ria; Rea??o em Cadeia da Polimerase / m?todos; Brasil / epidemiologia
Descrição
Foundation for Research Support of the State of SaoPaulo (FAPESP ? 06/1533-9) and the National Council for Scientific and Technological Development (CNPq ? Universal 470594/2006-3 and 475238/2008-7).
Universidade Federal de S?o Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. S?o Paulo, SP, Brazil.
Universidade Federal de S?o Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. S?o Paulo, SP, Brazil.
Universidade Federal de S?o Paulo. Departamento de Microbiologia, Imunologia e Parasitologia. S?o Paulo, SP, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universidade Federal do Esp?rito Santo. N?cleo de Doen?as Infecciosas. Vit?ria, ES, Brazil.
Universidade Federal do Esp?rito Santo. N?cleo de Doen?as Infecciosas. Vit?ria, ES, Brazil.
Universidade Federal do Esp?rito Santo. N?cleo de Doen?as Infecciosas. Vit?ria, ES, Brazil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brazil.
Funda??o Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Rio de Janeiro, RJ, Brazil.
Universidade Federal de Goi?s. Departamento de Microbiologia, Imunologia, Parasitologia e Patologia. Goi?nia, GO, Brazil.
Secretaria Municipal de Sa?de de Goi?nia. Goi?nia, GO, Brazil.
Secretaria Estadual da Sa?de do Paran?. Curitiba, PR, Brazil.
Laborat?rio Central de Sa?de P?blica. Porto Alegre, RS, Brazil.
Laborat?rio Central de Sa?de P?blica. Porto Alegre, RS, Brazil.
Centro de Vigil?ncia Epidemiol?gica Professor Alexandre Vranjac. S?o Paulo, SP, Brazil.
Centro de Vigil?ncia Epidemiol?gica Professor Alexandre Vranjac. S?o Paulo, SP, Brazil.
Instituto Adolfo Lutz. Setor de Micobact?rias. S?o Paulo, SP, Brazil.
Centro de Referencia Professor H?lio Fraga. Rio de Janeiro, RJ, Brazil.
Aim: Our aim is to investigate if the clusters of postsurgical mycobacterial infections, reported between 2004 and 2008 in seven geographically distant states in Brazil, were caused by a single mycobacterial strain. Materials & methods: Available information from 929 surgical patients was obtained from local health authorities. A total of 152 isolates from surgical patients were identified by PCR restriction enzyme analysis of the hsp65 gene (PRA-hsp65) and sequencing of the rpoB gene. Isolates were typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. A total of 15 isolates not related to surgical cases were analyzed for comparison. Results: All isolates were identified as Mycobacterium abscessus ssp. massiliense. Isolates from surgical patients and one sputum isolate grouped in a single PFGE cluster, composed of two closely related patterns, with one band difference. A total of 14 other isolates unrelated to surgical cases showed distinctive PFGE patterns. Conclusion: A particular strain of M. abscessus ssp. massiliense was associated with a prolonged epidemic of postsurgical infections in seven Brazilian states, suggesting that this strain may be distributed in Brazilian territory and better adapted to cause surgical-site infections.
