Autor(es): Vasconcelos, Pedro Fernando da Costa ; Rosa, Am?lia Paes de Andrade Travassos da ; Rodrigues, Sueli Guerreiro ; Tesh, Robert ; Rosa, Jorge Fernando Soares Travassos da ; Rosa, Elizabeth Salb? Travassos da
Data: 2017
Origem: Oasisbr
Assunto(s): Arenaviridae / patogenicidade; Infec??o Laboratorial / diagn?stico; Testes de Fixa??o de Complemento / m?todos; Ensaio de Imunoadsor??o Enzim?tica / m?todos; Arenaviridae / isolamento & purifica??o
Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Yale Arbovirus Research Unit, Yale University, New Haven, Connecticut, USA.
Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Funda??o Nacional de Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
S?o descritos os achados cl?nico-laboratoriais da infec??o acidental pelo virus SP H 114202 (Arenavirus, fam?lia Arenaviridae), um virus novo causador de febre hemorr?gica humana. O paciente, t?cnico de laborat?rio, apresentou quadro febril por 13 dias. A doen?a cursou com febre elevada ( 39?C.) di?ria, cefal?ia, calefrios e mialgias por 8 dias. A partir do 30 dia surgiram n?useas, v?mitos alimentares e anorexia eno 100 dia, epigastralgia, diarr?ia e gengivorragia. Laboratorialmente, foram observadas as seguintes altera??es: leucopenia gradativa com linfocitose a medida quea doen?a evoluia, passando de 5800 para 41 00,3400 e 2500 leucocitos por mmJ de sangue com 2, 5, 9 e 11 dias de doen?a, respectivamente. Ocorreram ainda, discretas altera??es nas transaminases (TGO=54 e TGP=52) no 90 dia de doen?a. O coagulograma e velocidade de hemossedimenta??o foram normais. Convers?o soro l?gica para o virus em apre?o, foi detectada atrav?s dos testes de fIXa??o do complemento, neutral?za??o e ELISA. Sangue do paciente inoculado em camundongos rec?m nascidos, hamsters e c?lulas VERO, apresentou resultado negativo para isolamento de virus. Esse virus foi isolado pela primeira vez em S?o Paulo, em 1990, a partir do sangue de um paciente que apresentou febre hemorr?gica com evolu??o fatal. Recomenda-sextremo cuidado no manuseio desse virus por tratar-se de pat? geno com riscos de transmiss?o por aeross?is (contaminante classe III ou IV).
Here in is described the clinical and laboratorial findings of a laboratory-acquired infection caused by the v?rus SP H 114202 (Arenavirus, family Arenaviridae) a recently discovered agent responsible for a viral hemorrhagic fever. The patient was sick for 13 days. The disease had an abrupt onset characterized by high fever (39?C.), headache, chills and myalgias for 8 days. In addition, on the 3rd day, the patient developed nauseas and vomiting, and in the 10th, epigastralgia, diarrheia and gengivorrhagia. Leucopenia was seen within the 1 st week of onset, with counts as low as 2,500 white cells per mm?. Counts perforrned after the 23th day of the onset were within norrnal limits. With the exception of moderate Iymphocitosis, no changes were observed in differential counts. An increase in lhe titer of antibodies by complement fixation, neutralization and ELISA (IgM) was detected. Suckling mice and baby hamsters were inoculated intracerebrally with 0.02 ml of blood samples collected in lhe 2nd and 7th days of disease. Attempts to isolate the virus were also made in Vero cells. No virus was isolated. Th?s v?rus was ?solated before ?n a single occasion ?n S?o Paulo State, in 1990, from the blood of a patient with hemorrhagic rever with a fatal outcome. The manipulation o f the virus under study, must be done carefully, since the transm?ssion can occur through aerosols.
