Autor(es): Mascarenhas, Joana D'Arc Pereira ; Bezerra, Delana A. M ; Silva, Ren? R ; Silva, Mayara J. M ; Sousa Junior, Edivaldo Costa ; Soares, Luana Souza
Data: 2017
Origem: Oasisbr
Assunto(s): Rotavirus; Infec??es por Rotavirus / epidemiologia; Aves Dom?sticas; Galinhas / virologia; Fezes / virologia; Ecossistema Amaz?nico; Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos; Eletroforese em Gel de Poliacrilamida / m?todos
This study was partially supported by a grant from Par? State Secretary of Science and Technology, contract no. 067/2008 (SEDECT/FAPESPA/PA).
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Agricultura. Laborat?rio Nacional Agropecu?rio. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
The aim of this study was to detect rotavirus F (RVF) and rotavirus G (RVG) in fecal specimens of broiler chickens in Brazil. During 2008 and 2011, a total of 85 fecal samples were collected. The viral genome was extracted, followed by polyacrylamide gel electrophoresis (PAGE), reverse transcription polymerase chain reaction (RT-PCR), and nucleotide sequencing. Samples were screened for rotaviruses by PAGE, and RVF and RVG genome banding patterns were not seen. Using RT-PCR, it was found that 9.4 % (8/85) of the pools contained RVF, whereas 10.6 % (9/85) contained RVG. The predicted amino acid sequences of RVF and RVG from Brazilian samples were 94.4-95.7 % and 96.8-96.9 % identical, respectively, to those of prototypes from Germany. The detection of RVF and RVG in this study provides important epidemiological data about the simultaneous circulation of rotaviruses affecting broiler flocks in the Amazon region of Brazil.
