Descrição
The work done at Institut Pasteur de la Guadeloupe was supported by a FEDER grant, financed by the European Union and Guadeloupe Region (Programme Op?rationnel FEDER-Guadeloupe-Conseil R?gional 2014?2020, Grant number 2015-FED-192). The ECC and IPF received support from Capes and FAPESPA respectively.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brazil.
Institut Pasteur de Guadeloupe. Unit? de la Tuberculose et des Mycobact?ries. WHO Supranational TB Reference Laboratory. Abymes, Guadeloupe, France.
Institut Pasteur de Guadeloupe. Unit? de la Tuberculose et des Mycobact?ries. WHO Supranational TB Reference Laboratory. Abymes, Guadeloupe, France.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universidade do Estado do Par?. Programa de P?s-gradua??o em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brazil.
Funda??o Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada as Micobact?rias. Rio de Janeiro, RJ, Brazil.
Funda??o Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada as Micobact?rias. Rio de Janeiro, RJ, Brazil.
Funda??o Oswaldo Cruz. Laborat?rio de Biologia Molecular Aplicada as Micobact?rias. Rio de Janeiro, RJ, Brazil / Institute of Tropical Medicine. Mycobacterium Unit. Antwerp, Belgium.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Polimorfismo de DNA. Bel?m, PA, Brazil.
Universidade Federal do Par?. N?cleo de Medicina Tropical. Bel?m, PA, Brazil.
Univ. Paris-Sud. Institut de Biologie Int?grative de la Cellule. Univ. Paris-Saclay, France.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia. Rio de Janeiro, RJ, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Par?. Programa de P?s-gradua??o em Biologia Parasit?ria na Amaz?nia. Bel?m, PA, Brazil.
There is only scarce information available on genotypic diversity of the Mycobacterium tuberculosis complex (MTBC) clinical isolates circulating in the Northern part of Brazil, a relatively neglected region regarding research on tuberculosis. We therefore characterized 980 MTBC clinical isolates from the state of Par?, by spoligotyping and data was compared with patterns from around the world, besides analyzing drug susceptibility, and collecting sociodemographic data. We also performed 24 loci MIRU-VNTR typing to evaluate phylogenetic inferences among the East-African-India (EAI) lineage strains. The Geographic Information System analyses were performed to generate a descriptive visualization of MTBC strain distribution in the region. A total of 249 different spoligopatterns primarily belonging to evolutionary recent Euro-American lineages, as well as Central-Asian, Manu and ancestral EAI lineages, were identified, in addition to strains with reportedly unknown lineage signatures. The most frequent lineages were Latin American Mediterranean, T and Haarlem. Interestingly, EAI lineage strains were found in a higher proportion in a significantly higher proportion in comparison with previous studies from South America. Regarding EAI lineage, the absence of spacers 4?9 and 23?24 co-related to 24 loci MIRU-VNTRs may suggest a close evolutionary relationship between such strains in Par? and those prevalent in Mozambique, which might have contributed to the genetic diversity of MTBC strains in this region.
