Autor(es):
Nunes Neto, Joaquim Pinto ; Souza, William Marciel de ; Acrani, Gustavo Olszanski ; Romeiro, Marilia Farignoli ; Fumagalli, Marc?lio Jorge ; Vieira, Luis Carlos ; Medeiros, Daniele Barbosa de Almeida ; Lima, Juliana Abreu ; Lima, Clayton Pereira Silva de ; Cardoso, Jedson Ferreira ; Figueiredo, Luiz Tadeu Moraes ; Silva, Sandro Patroca da ; Tesh, Robert ; Nunes, Marcio Roberto Teixeira ; Vasconcelos, Pedro Fernando da Costa
Data: 2017
Origem: Oasisbr
Assunto(s): Roedores / virologia; Xenartros / virologia; Psychodidae / virologia; Phlebovirus / classifica??o; Phlebovirus / gen?tica; Phlebovirus / isolamento & purifica??o; An?lise de Sequ?ncia de DNA; Homologia de Sequ?ncia; Varia??o Gen?tica; Genoma Viral; Filogenia; Brasil (BR)
Descrição
This work was supported by the Evandro Chagas Institute funds, Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) grants (301641/2010-2), and by the Funda?ao de Amparo ? Pesquisa do Estado de S?o Paulo, Brazil (grant numbers 13/14929-1 and 14/ 02438-6, and scholarships numbers 12/24150-9, 15/05778-5, 14/ 20851-8, 16/01414-1 and 14/15079-4), Universidade Federal do Par? and Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES).
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
University of S?o Paulo. School of Medicine of Ribeirao Preto. ?Virology Research Center. S?o Paulo, SP, Brazil / MRC-University of Glasgow. Centre for Virus Research. Glasgow, Scotland, UK.
Universidade Federal da Fronteira Sul. Passo Fundo, RS, Brazil.
University of S?o Paulo. School of Medicine of Ribeirao Preto. ?Virology Research Center. S?o Paulo, SP, Brazil
University of S?o Paulo. School of Medicine of Ribeirao Preto. ?Virology Research Center. S?o Paulo, SP, Brazil
University of S?o Paulo. School of Medicine of Ribeirao Preto. ?Virology Research Center. S?o Paulo, SP, Brazil
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??o Tecnol?gica. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??o Tecnol?gica. Bel?m, PA, Brasil.
University of S?o Paulo. School of Medicine of Ribeirao Preto. ?Virology Research Center. S?o Paulo, SP, Brazil
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??o Tecnol?gica. Bel?m, PA, Brasil.
University of Texas Medical Branch. Department of Pathology. Galveston TX, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??o Tecnol?gica. Bel?m, PA, Brazil / University of Texas Medical Branch. Department of Pathology. Galveston TX, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brazil / Par? State University. Department of Pathology. Bel?m, PA, Brazil.
The genus Phlebovirus includes the sandfly fever viruses and tick-transmitted uukuviruses. Sandfly fever group viruses have been isolated from various vertebrate species and from phlebotomines and occasionally alternative arthropods, e.g. mosquitoes, or ceratopogonids of the genus Culicoides. Uukuniemi serogroup viruses have been isolated from various vertebrate species and from ticks. Despite the public health importance of some viruses of the genus, the genomic diversity of phleboviruses that could be incriminated as causative of human or veterinary diseases remains underestimated. Here we describe the nearly complete sequences and genomic characterization of two phleboviruses belonging to the Bujaru antigenic complex: the prototype species and the Munguba virus. Furthermore, six previously unclassified phleboviruses isolated in Brazil were also sequenced and characterized: Ambe, Anhanga, Joa, Uriurana, Urucuri and Tapara viruses. The results of the phylogenetic analysis indicated that these viruses group with viruses of three antigenic complexes (Bujaru, Tapara and frijoles clades), with two unclassified phleboviruses. We also performed genomic reassortment analysis and confirmed that there were no events for the viruses described in this study, but we found a new potential reassortment in Medjerda Valley virus, which contains S and L segments of Arbia virus, and probably a unique M segment, both viruses circulate in the same geographic region, indicating these two isolates represent two distinct viruses. This study provides insights into the genetic diversity, classification and evolution of phleboviruses.
