Autor(es):
Menezes, Rubens Alex de Oliveira ; Gomes, Margarete do Socorro Mendon?a ; Mendes, Ana Paula Martins ; Couto, ?lvaro Augusto Ribeiro D?Almeida ; Nacher, Mathieu ; Pimenta, Tamirys Sim?o ; Sousa, Aline Collares Pinheiro de ; Baptista, Andrea regina de Souza ; Jesus, Maria Izabel de ; Enk, Martin Johannes ; Cunha, Maristela Gomes ; Machado, Ricardo Luiz Dantas
Data: 2018
Origem: Oasisbr
Assunto(s): Mal?ria Vivax / sangue; Mal?ria Vivax / complica??es; Mal?ria Vivax / epidemiologia; Doen?as Parasit?rias / sangue; Doen?as Parasit?rias / complica??es; Doen?as Parasit?rias / epidemiologia; Prote?nas de Protozo?rios / imunologia; Enteropatias Parasit?rias / epidemiologia; Estudos Transversais / m?todos; Citocinas / sangue; Coinfec??o / parasitologia; Ensaio de Imunoadsor??o Enzim?tica / m?todos; Citometria de Fluxo / m?todos; Imunoglobulina G / imunologia; Fezes / parasitologia; Helmint?ase / parasitologia; Brasil / epidemiologia; Guiana Francesa / epidemiologia
Descrição
Federal University of Para. Postgraduate Program in the Biology of Infectious and Parasitic Agents. Bel?m, PA, Brazil / Federal University of Amapa. Laboratory of morphofunctional and parasitic studies with impact on health. Macap?, AP, Brazil.
Amap? State Secretary of Health. Amap? Central Laboratory. Macap?, AP, Brazil.
Federal University of Amap? - Oiapoque Binational Campus. Oiapoque, AP, Brazil.
Amap? State Secretary of Health. Amap? Central Laboratory. Macap?, AP, Brazil.
Centre Hospitalier de Cayenne. Centre d?Investigation Clinique. Cayenne, French Guiana.
Federal University of Para. Postgraduate Program in Neuroscience and Cell Biology. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Fluminense Federal University. Niter?i, RJ, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Federal University of Para. Postgraduate Program in the Biology of Infectious and Parasitic Agents. Bel?m, PA, Brazil / Federal University of Para. Laboratory of Microbiology and Immunology. Bel?m, PA, Brazil.
Federal University of Para. Postgraduate Program in the Biology of Infectious and Parasitic Agents. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. / Fluminense Federal University, Nitero?i, Rio de Janeiro State, Brazil,
Malaria-enteroparasitic co-infections are known for their endemicity. Although they are prevalent, little is known about their epidemiology and effect on the immune response. This study evaluated the effect of enteroparasite co-infections with malaria caused by Plasmodium vivax in a border area between Brazil and French Guiana. The cross sectional study took place in Oiapoque, a municipality of Amap?, on the Amazon border. Malaria was diagnosed using thick blood smears, haemoglobin dosage by an automated method and coproparasitology by the Hoffman and Faust methods. The anti-PvMSP-119 IgG antibodies in the plasma were evaluated using ELISA and Th1 (IFN-?, TNF-? and IL-2), and Th2 (IL-4, IL-5 and IL-10) cytokine counts were performed by flow cytometry. The participants were grouped into those that were monoinfected with vivax malaria (M), vivax malaria-enteroparasite co-infected (CI), monoinfected with enteroparasite (E) and endemic controls (EC), who were negative for both diseases. 441 individuals were included and grouped according to their infection status: [M 6.9% (30/441)], [Cl 26.5% (117/441)], [E 32.4% (143/441)] and [EC 34.2% (151/441)]. Males prevailed among the (M) 77% (23/30) and (CI) 60% (70/117) groups. There was a difference in haemoglobin levels among the different groups under study for [EC-E], [EC-Cl], [E-M] and [Cl-M], with (p < 0.01). Anaemia was expressed as a percentage between individuals [CI-EC (p < 0.05)]. In terms of parasitaemia, there were differences for the groups [CI-M (p < 0.05)]. Anti-PvMSP-119 antibodies were detected in 51.2% (226/441) of the population. The level of cytokines evaluation revealed a large variation in TNF-? and IL-10 concentrations in the co-infected group. In this study we did not observe any influence of coinfection on the acquisition of IgG antibodies against PvMSP119, as well as on the profile of the cytokines that characterize the Th1 and Th2 patterns. However, co-infection increased TNF-? and IL-10 levels.
