Autor(es): Souza, William Marciel de ; Romeiro, Marilia Farignoli ; Sabino-Santos Jr, Gilberto ; Maia, Felipe Gon?alves Motta ; Fumagalli, Marc?lio Jorge ; Modha, Sejal ; Nunes, Marcio Roberto Teixeira ; Murcia, Pablo Ramiro ; Figueiredo, Luiz Tadeu Moraes
Data: 2018
Origem: Oasisbr
Assunto(s): Hepatite / diagn?stico; V?rus de Hepatite / isolamento & purifica??o; Roedores / virologia; Hepatite Viral Humana / virologia
This work was supported by the Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo, Brazil (Grant number. 13/14929-1, and Scholarships No. 17/13981-0; 12/24150-9; 15/05778-5; 14/20851-8; 16/01414-1; 06/00572-0; 08/06411-4 11/06810-9; 11/22663-6; 16/02568-2). PRM was supported by the Medical Research Council of the United Kingdom (Grant MC_UU_120/14/9).
University of S?o Paulo. Ribeir?o Preto Medical School. Virology Research Center. Ribeir?o Preto, SP, Brazil / MRC-University of Glasgow Centre for Virus Research. Glasgow, United Kingdom.
University of S?o Paulo. Ribeir?o Preto Medical School. Virology Research Center. Ribeir?o Preto, SP, Brazil.
University of S?o Paulo. Ribeir?o Preto Medical School. Virology Research Center. Ribeir?o Preto, SP, Brazil.
University of S?o Paulo. Ribeir?o Preto Medical School. Virology Research Center. Ribeir?o Preto, SP, Brazil / University of S?o Paulo. Laboratory Institute of Biomedical Sciences. S?o Paulo, SP, Brazil.
University of S?o Paulo. Ribeir?o Preto Medical School. Virology Research Center. Ribeir?o Preto, SP, Brazil.
MRC-University of Glasgow Centre for Virus Research. Glasgow, United Kingdom.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil.
MRC-University of Glasgow Centre for Virus Research. Glasgow, United Kingdom.
University of S?o Paulo. Ribeir?o Preto Medical School. Virology Research Center. Ribeir?o Preto, SP, Brazil.
The Hepeviridae comprise single-stranded positive-sense RNA viruses classified into two genera, Orthohepevirus and Piscihepevirus. Orthohepeviruses have a wide host range that includes rodents, but previous studies had been restricted to rodents of the Muridae family. In this study, we applied a high-throughput sequencing approach to examine the presence of orthohepeviruses in rodents from S?o Paulo State, Brazil. We also used RT-PCR to determine the frequency of orthohepeviruses in our sampled population. We identified novel orthohepeviruses in blood samples derived from Necromys lasiurus (1.19%) and Calomys tener (3.66%). Therefore, our results expand the host range and viral diversity of the Hepeviridae family.
