Autor(es): Freitas, Ronaldo Barros de ; Melo, Fernando L ; Oliveira, Darleise de Souza ; Romano, Camila M ; Freitas, Maria Rute Castro ; Mac?do, Olinda ; Linhares, Alexandre da Costa ; Zanotto, Paolo M. de A ; Durigon, Edison Luiz
Data: 2018
Origem: Oasisbr
Assunto(s): Erythrovirus / isolamento & purifica??o; Filogenia; Parvovirus B19 Humano; Eritema Infeccioso
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Universidade de S?o Paulo. Instituto de Ci?ncias Biom?dicas. Departamento de Microbiologia. Laborat?rio de Evolu??o Molecular e Bioinform?tica. S?o Paulo, SP, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Universidade de S?o Paulo. Instituto de Ci?ncias Biom?dicas. Departamento de Microbiologia. Laborat?rio de Evolu??o Molecular e Bioinform?tica. S?o Paulo, SP, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Bel?m, PA, Brasil.
Universidade de S?o Paulo. Instituto de Ci?ncias Biom?dicas. Departamento de Microbiologia. Laborat?rio de Evolu??o Molecular e Bioinform?tica. S?o Paulo, SP, Brazil.
Universidade de S?o Paulo. Instituto de Ci?ncias Biom?dicas. Departamento de Microbiologia. Laborat?rio de Virologia Cl?nica. S?o Paulo, SP, Brazil.
Background: Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations.Objectives: To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients inthe Amazon region with various clinical presentations.Study design: A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested usingspecific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction.Results: B19 DNA was detected in 117 (24 por cento) of 487 samples. Of these, 106 (91 por cento) isolates were genotype 1 and 11 (9 por cento) were genotype 3.No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may havebeen transmitted via transfusion of blood products.Conclusion: We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years.
