Descrição
This work was supported by Rede de Pesquisa em Gen?mica Populacional Humana (RPGPH)?3381/2013 CAPES-BioComputacional; FADESP/PROPESP/UFPA (Universidade Federal do Par?); and CNPq (Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico). It was also supported by CNPq/Produtividade grant 304413/2015-1 to A.R.S. and CNPq/Produtividade grant 305258/2013-3 to S.S.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Gen?tica Humana e M?dica. Bel?m, PA, Brazil.
Universidade Federal do Rio Grande do Norte. Bioinformatics Multidisciplinary Environment. Natal, RN, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Gen?tica Humana e M?dica. Bel?m, PA, Brazil.
Universidade Federal do Rio Grande do Norte. Bioinformatics Multidisciplinary Environment. Natal, RN, Brazil.
Universidade Federal do Rio Grande do Norte. Bioinformatics Multidisciplinary Environment. Natal, RN, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Gen?tica Humana e M?dica. Bel?m, PA, Brazil / Universidade Federal do Rio Grande do Norte. Departmento de Gen?tica. Natal, RN, Brazil.
Instituto Nacional de C?ncer. Programa de Gen?tica. Rio de Janeiro, RJ, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Bioqu?mica e Imunologia. Belo Horizonte, MG, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Cultura de Tecidos e Citogen?tica. Ananindeua, PA, Brasil.
Universidade Federal Rural da Amaz?nia. Laborat?rio de Gen?tica Aplicada. Bel?m, PA, Brazil.
Universidade Federal do Par?. N?cleo de Pesquisas em Oncologia. Bel?m, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Gen?tica Humana e M?dica. Bel?m, PA, Brazil / Universidade Federal do Par?. N?cleo de Pesquisas em Oncologia. Bel?m, PA, Brazil.
Universidade Federal do Rio Grande do Norte. Bioinformatics Multidisciplinary Environment. Natal, RN, Brazil / Universidade Federal do Rio Grande do Norte. Departamento de Bioqu?mica. Natal, RN, Brazil.
Instituto Nacional de C?ncer. Programa de Gen?tica. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Departamento de Gen?tica. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Rio Grande do Norte. Bioinformatics Multidisciplinary Environment. Natal, RN, Brazil / Universidade Federal do Rio Grande do Norte. Instituto do C?rebro. Natal, RN, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Gen?tica Humana e M?dica. Bel?m, PA, Brazil / Universidade Federal do Par?. N?cleo de Pesquisas em Oncologia. Bel?m, PA, Brazil.
The Pirarucu (Arapaima gigas) is one of the world?s largest freshwater fishes and member of the superorder Osteoglossomorpha (bonytongues), one of the oldest lineages of ray-finned fishes. This species is an obligate air-breather found in the basin of the Amazon River with an attractive potential for aquaculture. Its phylogenetic position among bony fishes makes the Pirarucu a relevant subject for evolutionary studies of early teleost diversification. Here, we present, for the first time, a draft genome version of the A. gigas genome, providing useful information for further functional and evolutionary studies. The A. gigas genome was assembled with 103-Gb raw reads sequenced in an Illumina platform. The final draft genome assembly was ?661?Mb, with a contig N50 equal to 51.23?kb and scaffold N50 of 668?kb. Repeat sequences accounted for 21.69% of the whole genome, and a total of 24,655 protein-coding genes were predicted from the genome assembly, with an average of nine exons per gene. Phylogenomic analysis based on 24 fish species supported the postulation that Osteoglossomorpha and Elopomorpha (eels, tarpons, and bonefishes) are sister groups, both forming a sister lineage with respect to Clupeocephala (remaining teleosts). Divergence time estimations suggested that Osteoglossomorpha and Elopomorpha lineages emerged independently in a period of ?30 Myr in the Jurassic. The draft genome of A. gigas provides a valuable genetic resource for further investigations of evolutionary studies and may also offer a valuable data for economic applications.
