Author(s):
Xie, Xuping ; Kum, Dieudonn? B ; Xia, Hongjie ; Luo, Huanle ; Shan, Chao ; Zou, Jing ; Muruato, Antonio E ; Medeiros, Daniele Barbosa de Almeida ; Nunes, Bruno Tardelli Diniz ; Dallmeier, Kai ; Rossi, Shannan L ; Weaver, Scott C ; Neyts, Johan ; Wang, Tian ; Vasconcelos, Pedro Fernando da Costa ; Shi, Pei-Yong
Date: 2018
Origin: Oasisbr
Subject(s): Vacinas / imunologia; Zika virus / patogenicidade; Vacinas / antagonistas & inibidores; Infec??o pelo Zika virus; Prote?nas do Caps?deo; Ensaio Cl?nico; Estudos de Valida??o
Description
The P.-Y.S. lab was supported by a Kleberg Foundation Award and NIH grant AI127744. This research was also partially supported by NIH grant AI120942 to S.C.W. and by Cooperative Agreement Number U01CK000512 to S.C.W., funded by the CDC
University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.
University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Virology and Chemoth. KU Leuven. Leuven, Belgium.
University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.
University of Texas Medical Branch. Department of Microbiology & Immunology. Galveston, TX, USA.
University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.
University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.
University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA.
University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Virology and Chemoth. KU Leuven. Leuven, Belgium.
University of Texas Medical Branch. Institute for Human Infections & Immunity. Galveston, TX, USA / University of Texas Medical Branch. Department of Pathology and Center for Biodefense & Emerging Infectious Diseases. Galveston, TX, USA.
University of Texas Medical Branch. Institute for Human Infections & Immunity. Galveston, TX, USA / University of Texas Medical Branch. Institute for Translational Sciences. Galveston, TX, USA / University of Texas Medical Branch. Department of Microbiology & Immunology. Galveston, TX, USA / University of Texas Medical Branch. Department of Pathology and Center for Biodefense & Emerging Infectious Diseases. Galveston, TX, USA / University of Texas Medical Branch. Sealy Institute for Vaccine Sciences. Galveston, TX, USA.
University of Leuven. Rega Institute for Medical Research. Department of Microbiology and Immunology. Laboratory of Virology and Chemoth. KU Leuven. Leuven, Belgium.
University of Texas Medical Branch. Department of Microbiology & Immunology. Galveston, TX, USA / University of Texas Medical Branch. Department of Pathology and Center for Biodefense & Emerging Infectious Diseases. Galveston, TX, USA / University of Texas Medical Branch. Sealy Institute for Vaccine Sciences. Galveston, TX, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Par? State University. Department of Pathology. Bel?m, PA, Brazil.
University of Texas Medical Branch. Department of Biochemistry & Molecular Biology. Galveston, TX, USA / University of Texas Medical Branch. Sealy Institute for Vaccine Sciences. Galveston, TX, USA / University of Texas Medical Branch. Sealy Center for Structural Biology & Molecular Biophysics. Galveston, TX, USA / University of Texas Medical Branch. Department of Pharmacology & Toxicology. Galveston, TX, USA.
Zika virus (ZIKV) infection of the mother during pregnancy causes devastating Zika congenital syndrome in the offspring. A ZIKV vaccine with optimal safety and immunogenicity for use in pregnant women is critically needed. Toward this goal, we have developed a single-dose live-attenuated vaccine candidate that infects cells with controlled, limited infection rounds. The vaccine contains a 9-amino-acid deletion in the viral capsid protein and replicates to titers of > 10 6 focus-forming units (FFU)/mL in cells expressing the full-length capsid protein. Immunization of A129 mice with one dose (10 5 FFU) did not produce viremia, but elicited protective immunity that completely prevented viremia, morbidity, and mortality after challenge with an epidemic ZIKV strain (10 6 PFU). A single-dose vaccination also fully prevented infection of pregnant mice and maternal-to-fetal transmission. Intracranial injection of the vaccine (10 4 FFU) to 1-day-old mice did not cause any disease or death, underscoring the safety of this vaccine candidate.
