Author(s):
Portal, Thayara Morais ; Reym?o, Tammy Kathlyn Amaral ; Quinder? Neto, Germano Alves ; Fiuza, Mercedes Kamila da C ; Teixeira, Dielle Monteiro ; Lima, Ian Carlos Gomes ; Sousa J?nior, Edivaldo Costa ; Bandeira, Renato da Silva ; Deus, Danielle Rodrigues de ; Justino, Maria Cleonice Aguiar ; Linhares, Alexandre da Costa ; Silva, Luciana Damascena da ; Resque, Hugo Reis ; Gabbay, Yvone Benchimol
Date: 2018
Origin: Oasisbr
Subject(s): Gastroenterite / patologia; Infec??es por Adenovirus Humanos; Adenov?rus Humanos / patogenicidade; Sapovirus; Mamastrovirus; Diarreia Infantil / patologia; Rea??o em Cadeia da Polimerase / m?todos
Description
This research was supported by Evandro Chagas Institute; Capes.
University of State of Par?. Postgraduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas.Program de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil.
University of State of Par?. Undergraduate in Nursing. Bel?m, PA, Brazil.
School of the Amazon. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
University of State of Par?. Postgraduate Program in Parasitic Biology in the Amazon. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples and sera were collected from children hospitalized for acute gastroenteritis (AGE) in two large pediatric hospitals in Bel?m, from March 2012 to April 2015. The samples were analyzed by polymerase chain reaction (PCR) for adenovirus (AdV) and HAstV (astrovirus) detection, and Nested-PCR and qPCR for sapovirus (SaV) detection. AdV was detected in 50.2% (110/219) of the cases, with 42.7% (47/110) being sequenced and classified as: species F (63.9% - 30/47), A (4.2% - 2/47), B (6.4% - 3/47), C (17.1% - 8/47), D (4.2% - 2/47), and E (4.2% - 2/47). Of the 110 AdV-positive faeces samples, 80 paired sera presented sufficient amounts and were also tested for this virus, of which 51 (63.7%) showed positive results and 26 (70.3%) pairs (feces plus sera) presented concordant results after sequencing being classified as: species F (21/26; 80.8%), A (1/26; 3.8%), B (1/26; 3.8%), and C (3/26; 11.5%). Overall, HAstV rate in the feces samples was 1.8% (4/219), including both HAstV-1a (2/4; 50%) and HAstV-2c (2/4; 50%). SaV was detected in 4.6% (10/219) of the fecal samples, out of which 50% (5/10) of the positive samples were characterized into the genogroups GI.1 (1), GI.2 (2), and GII.4 (2). These findings highlighted the important contributions of AdV, HAstV, and SaV in the enteric virus spectrum in our region and showed the high genetic diversity of AdV. In addition, it demonstrated for the first time in Brazil, the circulation of AdV in the serum of hospitalized children with AGE.
