Autor(es): Almeida, Silvana de F. Oliveira de ; Silva, Let?cia R. Carvalho ; Chagas Junior, Gilson Celso A ; Oliveira, Guilherme ; Silva, Silvia Helena Marques da ; Vasconcelos, Santelmo ; Lopes, Alessandra Santos
Data: 2019
Origem: Oasisbr
Assunto(s): Cacau / microbiologia; Alimenta??o; Fermenta??o; Leveduras / crescimento & desenvolvimento; Leveduras / gen?tica; Sementes / gen?tica; Saccharomyces cerevisiae; Pichia; Medicil?ndia (PA); Tucum? (PA)
This study was financed by the Instituto Tecnol?gico Vale (ITV) (CacauP2). Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq (proc. 309312/2012-4).
Universidade Federal do Par?. Programa de P?s-gradua??o em Ci?ncia e Tecnologia de Alimentos. Laborat?rio de Processos Biotecnol?gicos. Bel?m, PA, Brazil.
Universidade Federal do Par?. Programa de P?s-gradua??o em Ci?ncia e Tecnologia de Alimentos. Laborat?rio de Processos Biotecnol?gicos. Bel?m, PA, Brazil.
Universidade Federal do Par?. Programa de P?s-gradua??o em Ci?ncia e Tecnologia de Alimentos. Laborat?rio de Processos Biotecnol?gicos. Bel?m, PA, Brazil.
Instituto Tecnol?gico Vale. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Micologia. Ananindeua, PA, Brasil.
Instituto Tecnol?gico Vale. Bel?m, PA, Brazil.
Universidade Federal do Par?. Programa de P?s-gradua??o em Ci?ncia e Tecnologia de Alimentos. Laborat?rio de Processos Biotecnol?gicos. Bel?m, PA, Brazil.
The purpose of this study was to identify the yeasts involved in spontaneous fermentation of cocoa from the Brazilian Amazon region. The fermentation process was carried out experimentally with cocoa seeds from two sites (Medicil?ndia and Tucum?), State of Par?, northern Brazil, during a six-day period. Totals of 44 yeasts were isolated from Medicil?ndia and 29 from Tucum?. Molecular identification was carried out by sequencing the D1/D2 region fragment of the rRNA 26S gene, expanded with universal primers for the NL1GC and LS2 eukaryotes. Pichia manshurica and Saccharomyces cerevisiae were identified in Medicil?ndia and five yeast species (Pichia fermentans, P. kudriavzevii, P. manshurica, S. cerevisiae and Zygosaccharomyces bailii) were identified in Tucum?. The results showed that P. manshurica and S. cerevisiae may have potential for use as starter cultures in future studies to improve the quality of cocoa seeds fermented in the Brazilian Amazon region.
