Author(s): Chapadense, Francesca ; Machado, Ricardo Luiz Dantas ; Ventura, Ana Maria Revor?do da Silva ; ?reas, Andr? ; Machado, Renato Beilner ; Vianna, Giselle Maria Rachid ; Zalis, Mariano Gustavo ; Castro, Ana Maria de ; Cravo, Pedro
Date: 2019
Origin: Oasisbr
Subject(s): Mal?ria / parasitologia; Plasmodium falciparum / patogenicidade; Rea??o em Cadeia da Polimerase / m?todos; Resist?ncia a Medicamentos / efeitos dos f?rmacos; Antimal?ricos; Parasitos / parasitologia
This project received partial federal support from the Evandro Chagas Institute/Brazilian Ministry of Health. This research was also partially financed by FAPESP (S?o Paulo Research Foundation) through Grant 02/9546-1 and CNPq (National Council for Scientific and Technological Development) through Grant 302353/2003-8
Universidade Federal de Goi?s. Instituto de Patologia Tropical e Sa?de P?blica. Goi?nia, GO, Brasil.
Universidade Federal de Fluminense. Departamento de Microbiologia e Parasitologia. Niter?i, RJ, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universit?rio Clementino Fraga Filho. Laborat?rio de Infectologia e Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universit?rio Clementino Fraga Filho. Laborat?rio de Infectologia e Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Goi?s. Instituto de Patologia Tropical e Sa?de P?blica. Goi?nia, GO, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universidade Federal do Rio de Janeiro. Hospital Universit?rio Clementino Fraga Filho. Laborat?rio de Infectologia e Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade Federal de Goi?s. Instituto de Patologia Tropical e Sa?de P?blica. Goi?nia, GO, Brasil.
Universidade Nova de Lisboa. Instituto de Higiene e Medicina Tropical. Lisboa, Portugal / UniEvang?lica. Centro Universit?rio de An?polis. An?polis, GO, Brasil.
INTRODUCTION: Mutations in the propeller domain of the Plasmodium falciparum kelch13 (k13) gene are associated with artemisinin resistance. METHODS: We developed a PCR protocol to sequence the pfk13 gene and determined its sequence in a batch of 50 samples collected from 2003 to 2016 in Brazil. RESULTS: We identified 1 K189T substitution located outside the propeller domain of the PfK13 protein in 36% of samples. CONCLUSIONS: Although the sample size is relatively small, these results suggest that P. falciparum artemisinin-resistant mutants do not exist in Brazil, thereby supporting the continuation of current treatment programs based on artemisinin-based combination therapy.
