Author(s):
Silva, Tatiene R. M ; Barros, Fl?via N. L ; Bahia, Michele ; Sampaio Junior, Francisco D ; Santos, Sidney S. F ; Inoue, Larissa S ; Gon?alves, Thamirys S ; Chiesorin Neto, Laerzio ; Faria, Diogo C. L. O ; Tchetto, Camila ; Viana, Giselle Maria Rachid ; Monteiro, Frederico O. B ; Cavalcante, Gustavo G?es ; Scofield, Alessandra
Date: 2019
Origin: Oasisbr
Subject(s): Plasmodium vivax / patogenicidade; Plasmodium falciparum / patogenicidade; Mal?ria / veterin?ria; Primatas / anatomia & histologia; Primatas / parasitologia; Monitoramento Epidemiol?gico / veterin?ria; Rea??o em Cadeia da Polimerase / m?todos; Amaz?nia Brasileira (BR)
Description
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
Federal Rural University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
Sauim Castanheiras Wildlife Refuges. Manaus, AM, Brazil.
Chico Mendes Institute for Biodiversity Conservation. Manaus, AM, Brazil.
Jungle Warfare Instruction Center, Manaus, Brazil
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Pesquisa B?sica em Mal?ria. Ananindeua, PA, Brasil.
Federal Rural University of the Amazon. Institute of Veterinary Medicine. Postgraduate Program in Health and Animal Production in the Amazon. Bel?m, PA, Brazil.
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
Federal University of Par?. Institute of Veterinary Medicine. Postgraduate Program in Animal Health in the Amazon. Laboratory of Animal Parasitology. Castanhal, PA, Brazil.
The Brazilian Amazon is endemic for malaria and natural infections by Plasmodium spp. have been detected in Neotropical primates. Despite the diversity of primate species in the region, studies on infections by these agents are limited. The aim of the present study was to investigate the frequency of infection by Plasmodium vivax and P. falciparum in free-born primates that were kept in captivity, in the western Amazon, Brazil. Blood samples were collected from 98 Neotropical primates. Detection of P. vivax and P. falciparum DNA was performed using a semi-nested PCR, and the amplified products were sequenced. Plasmodium spp. DNA was detected in 6.12% (6/98) of the primates. P. vivax, and P. falciparum DNA was detected in 2.04% (2/98) and 4.08% (4/98) of these mammals, respectively. Sequencing and phylogenetic analysis confirmed the results obtained from the semi-nested PCR. The presence of infected non-human primates (NHP) can be auxiliary in the maintenance of P. falciparum and P. vivax and may have implications for the malaria surveillance and control in the Brazilian Amazon. It is necessary to structure an efficient surveillance system for the aetiological agents of malaria that infect NHP and humans to reduce the risk of Plasmodium spp. introduction into new areas, to protect all susceptible species.
