Autor(es):
Lima, Alex Ranieri Jer?nimo ; Siqueira, Andrei Santos ; Vasconcelos, Janaina Mota de ; Pereira, James Siqueira ; Azevedo, Juliana Sim?o Nina de ; Moraes, Pablo Henrique Gon?alves ; Aguiar, D?lia Cristina Figueira ; Lima, Clayton Pereira Silva de ; Vianez J?nior, Jo?o L?dio da Silva Gon?alves ; Nunes, M?rcio Roberto Teixeira ; Xavier, Luciana Pereira ; Dall?Agnol, Leonardo Teixeira ; Gon?alves, Evonnildo Costa
Data: 2019
Origem: Oasisbr
Assunto(s): Cianobact?rias / gen?tica; Genoma / gen?tica; An?lise de Sequ?ncia de DNA; Limnothrix spp; Metabolismo / gen?tica; Ecossistema Amaz?nico; Centrais Hidrel?tricas
Descrição
This work was funded by Brazilian Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico ? CNPq (grants 556851/2009-9 and 554321/2010-6) and Funda??o Amaz?nia Paraense (FAPESPA) (grant ICAAF 006/2012). This work was financially supported through grants from CAPES (Coordination for the Improvement of Higher Education Personnel, Brazil), PROPESP/UFPA (Pr?-Reitoria de Pesquisa e P?s-Gradua??o/Universidade Federal do Par?, Brazil), FAPEMA (Maranh?o State Research Foundation, Brazil), and Instituto Evandro Chagas (IEC).
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil.
Universidade Federal Rural da Amaz?nia - Campus de Capanema. Laborat?rio de Biodiversidade Molecular. Capanema, PA, Brazil.
Universidade Federal Rural da Amaz?nia - Campus de Capanema. Laborat?rio de Biodiversidade Molecular. Capanema, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Biotecnologia de Enzimas e Biotransforma??es. Bel?m, PA, Brazil.
Universidade Federal do Maranh?o. Grupo de Pesquisa em Biodiversidade, Bioprospec??o e Biotecnologia. S?o Lu?s, MA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Laborat?rio de Tecnologia Biomolecular. Bel?m, PA, Brazil.
Currently only four genome sequences for Limnothrix spp. are publicly available, and information on the genetic properties of cyanobacteria belonging to this genus is limited. In this study, we report the draft genome of Limnothrix sp. CACIAM 69d, isolated from the reservoir of a hydroelectric dam located in the Amazon ecosystem, from where cyanobacterial genomic data are still scarce. Comparative genomic analysis of Limnothrix revealed the presence of key enzymes in the cyanobacterial central carbon metabolism and how it is well equipped for environmental sulfur and nitrogen acquisition. Additionally, this work covered the analysis of Limnothrix CRISPR-Cas systems, pathways related to biosynthesis of secondary metabolites and assembly of extracellular polymeric substances and their exportation. A trans-AT PKS gene cluster was identified in two strains, possibly related to the novel toxin Limnothrixin biosynthesis. Overall, the draft genome of Limnothrix sp. CACIAM 69d adds new data to the small Limnothrix genome library and contributes to a growing representativeness of cyanobacterial genomes from the Amazon region. The comparative genomic analysis of Limnothrix made it possible to highlight unique genes for each strain and understand the overall features of their metabolism.
