Autor(es):
Nunes, M?rcio Roberto Teixeira ; Souza, William Marciel de ; Savji, Nazir ; Figueiredo, M?rio Lu?s ; Cardoso, Jedson Ferreira ; Silva, Sandro Patroca da ; Lima, Clayton Pereira Silva de ; Vasconcelos, Helena Baldez ; Rodrigues, Sueli Guerreiro ; Lipkin, W. Ian ; Vasconcelos, Pedro Fernando da Costa ; Palacios, Gustavo
Data: 2019
Origem: Oasisbr
Assunto(s): Arbovirus / isolamento & purifica??o; Orthobunyavirus / patogenicidade; Arbov?rus / gen?tica; Gen?tipo; Rea??o em Cadeia da Polimerase / m?todos; Rearranjo G?nico
Descrição
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Texas Medical Branch. Center for Biodefense and Emerging Infectious Diseases. Department of Pathology. Galveston, TX, USA.
University of S?o Paulo. School of Medicine of Ribeirao Preto. Virology Research Center. Ribeirao Preto, SP, Brazil / MRC-University of Glasgow Centre for Virus Research. Glasgow, Scotland, United Kingdom.
Massachusetts General Hospital. Department of Medicine. Boston, MA, USA.
University of S?o Paulo. Faculty of Pharmaceutical Sciences of Ribeir?o Preto. Ribeir?o Preto, SP, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Columbia University. Mailman School of Public Health. Center for Infection and Immunity. New York, NY, USA.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil
US Army Medical Research Institute of Infectious Disease at Fort Detrick. Center for Genome Science. MD, USA.
Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Par? and Maranh?o states, nine as genotype II from Par? and Rond?nia states confirmed, four classified into genotype III from Acre, Maranh?o, Minas Gerais and Rond?nia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections.
