Author(s):
Ferreira, J?ssica da Silva ; Carvalho, Fernanda Marques de ; Pessolani, Maria Cristina Vidal ; Antunes, Jo?o Marcelo Azevedo de Paula ; Oliveira, Ilanna Vanessa Pristo de Medeiros ; Moura, Gabriela H?mylin Ferreira ; Truman, Richard Wayne ; Pe?a, Maria Tereza ; Sharma, Rahul ; Duthie, Malcolm S ; Guimar?es, Ricardo Jos? de Paula Souza e ; Fontes, Amanda Nogueira Brum ; Suffys, Philip Noel ; McIntosh, Douglas
Date: 2019
Origin: Oasisbr
Subject(s): Hansen?ase / diagn?stico; Hansen?ase / transmiss?o; Mycobacterium leprae / patogenicidade; Euphractus sexcinctus; Tatu; Zoonoses
Description
Federal Rural University of Rio de Janeiro. Institute of Veterinary Medicine. Department of Animal Parasitology. Multiuser Molecular Biology Laboratory. Serop?dica, RJ, Brazil.
Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Cellular Microbiology. Rio de Janeiro, RJ, Brazil.
Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Cellular Microbiology. Rio de Janeiro, RJ, Brazil.
Federal Rural University of Semi-?rido. Hospital Veterinary. Rio Grande do Norte, RN, Brazil.
Federal Rural University of Semi-?rido. Hospital Veterinary. Rio Grande do Norte, RN, Brazil.
Federal Rural University of Semi-?rido. Hospital Veterinary. Rio Grande do Norte, RN, Brazil.
National Hansen's Disease Program. Healthcare Systems Bureau. Health Resources and Services Administration. Department of Health and Humans Services. Baton Rouge, United States.
National Hansen's Disease Program. Healthcare Systems Bureau. Health Resources and Services Administration. Department of Health and Humans Services. Baton Rouge, United States.
National Hansen's Disease Program. Healthcare Systems Bureau. Health Resources and Services Administration. Department of Health and Humans Services. Baton Rouge, United States.
Infectious Disease Research Institute. Seattle, United States.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Geoprocessamento. Ananindeua, PA, Brasil.
Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Molecular Biology Applied to Mycobacteria. Rio de Janeiro, RJ, Brazil.
Funda??o Oswaldo Cruz. Oswaldo Cruz Institute. Laboratory of Molecular Biology Applied to Mycobacteria. Rio de Janeiro, RJ, Brazil.
Federal Rural University of Rio de Janeiro. Institute of Veterinary Medicine. Department of Animal Parasitology. Multiuser Molecular Biology Laboratory. Serop?dica, RJ, Brazil.
Leprosy was recognized as a zoonotic disease, associated with nine-banded armadillos (Dasypus novemcinctus) in the Southern United States of America in 2011. In addition, there is growing evidence to support a role for armadillos in zoonotic leprosy in South America. The current study evaluated twenty specimens of the six-banded armadillo (Euphractus sexcinctus), collected from rural locations in the state of Rio Grande do Norte (RN), Brazil for evidence of infection with Mycobacterium leprae. Serum was examined using two "in-house" enzyme-linked immunosorbent assays (ELISAs) and via two commercially available (ML flow and NDO-LID?) immunochromatographic lateral flow (LF) tests, for detection of the PGL-I and/or LID-1 antigens of the bacterium. The presence of M. leprae DNA in liver tissue was examined using the multi-copy, M. leprae-specific repetitive element (RLEP), as target in conventional and nested PCR assays. Molecular and anti-PGL-I-ELISA data indicated that 20/20 (100 %) of the armadillos were infected with M. leprae. The corresponding detection levels recorded with the LF tests were 17/20 (85 %) and 16/20 (85 %), for the NDO-LID? and ML flow tests, respectively. Our results indicate that, in common with D. novemcinctus, six banded armadillos (a species hunted and reared as a food-source in some regions of Brazil, including RN), represent a potential reservoir of M. leprae and as such, their role in a possible zoonotic cycle of leprosy within Brazil warrants further investigation.
