Autor(es):
Malossi, Camila Dantas ; Fioratti, Eduardo Gorzoni ; Cardoso, Jedson Ferreira ; Magro, Angelo Jos? ; Kroon, Erna Geessien ; Aguiar, Daniel Moura de ; Borges, Alice Mamede Costa Marques ; Nogueira, Marcia Furlan ; Ullmann, Leila Sabrina ; Araujo Jr, Jo?o Pessoa
Data: 2020
Origem: Oasisbr
Assunto(s): Anemia / veterin?ria; Lentivirus Equinos / isolamento & purifica??o; Lentivirus Equinos / patogenicidade; V?rus da Anemia Infecciosa Equina / gen?tica; Genoma Viral / gen?tica; Cavalos / virologia; Sequenciamento Completo do Genoma / veterin?ria; Pantanal (MT)
Descrição
Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo (2014/13532-3).
S?o Paulo State University. Institute for Biotechnology. Botucatu, SP, Brazil.
Vales do Jequitinhonha e Mucuri Federal University. Agrarian Sciences Institute. Una?, MG, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil
S?o Paulo State University. Institute for Biotechnology. Botucatu, SP, Brazil.
Universidade Federal de Minas Gerais. Virology Laboratory. Belo Horizonte, MG, Brasil.
Mato Grosso Federal University. Virology and Rickettsiosis Laboratory. Cuiab?, GO, Brazil.
Mato Grosso Federal University. Virology and Rickettsiosis Laboratory. Cuiab?, GO, Brazil.
EMBRAPA Pantanal. Corumb?, MT, Brazil.
S?o Paulo State University. Institute for Biotechnology. Botucatu, SP, Brazil.
S?o Paulo State University. Institute for Biotechnology. Botucatu, SP, Brazil.
Abstract: Equine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectious anemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory in this area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAV are currently available only for gag and LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic area in Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing (NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning, Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilian strain comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions
