Autor(es):
Costa, Emanuel Ramos da ; Henrique, Ediely Pereira ; Silva, Jo?o Batista da ; Pereira, Patrick Douglas Corr?a ; Abreu, Cintya Castro de ; Fernandes, Taiany Nogueira ; Magalh?es, Nara Gyzely de Morais ; Silva, Anderson de Jesus Falc?o da ; Guerreiro, Luma Cristina Ferreira ; Diniz, Cristovam Guerreiro ; Diniz, Cristovam Wanderley Pican?o ; Diniz, Daniel Guerreiro
Data: 2020
Origem: Oasisbr
Assunto(s): Charadriiformes / anatomia & histologia; Aves; Sistema Nervoso / an?lise; Astr?citos / citologia; Hipocampo / citologia; Voo Animal; Ruddy turnstone (Arenaria interpres)
Descrição
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brazil.
Instituto Federal de Educa??o. Ci?ncia e Tecnologia do Par?. Laborat?rio de Biologia Molecular e Neuroecologia. Bragan?a, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brazil.
Universidade Federal do Par?. Instituto de Ci?ncias Biol?gicas. Hospital Universit?rio Jo?o de Barros Barreto. Laborat?rio de Investiga??es em Neurodegenera??o e Infec??o. Bel?m, PA, Brazil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Microscopia Eletr?nica. Bel?m, PA, Brasil.
Astrocytes are essential for lipid neuronal metabolism in long-distance uninterrupted migratory flights, when glucose is not available as the main source of energy. We previously demonstrated in Calidris pusilla that after uninterrupted 5 days transatlantic flight, astrocytes shrink and reduce its number in the hippocampal formation. Here we shifted our attention to the wintering period and tested the hypothesis that hippocampal astrocyte morphology of A interpres will change as the wintering period progresses towards the premigration window. To that end we used Arenaria interpres, which also crosses the Atlantic Ocean and reaches the mangroves of the Amazon River estuary for wintering. Birds were captured in September/October (closer to the arrival in the coast of Bragan?a, Para, Brazil for wintering) and in April/May (closer to the departure towards the breeding sites) and had their brains processed for selective GFAP-astrocyte immunolabeling. Three-dimensional reconstructions of the immunostained astrocytes were performed and morphological classification was done based on hierarchical cluster and discriminant analysis of multimodal morphometric features. We found two morphological phenotypes of astrocytes in the newcomers which differentially increased its morphological complexities as wintering period progresses towards the pre-migration window. Taken together, our findings demonstrate that the long-distance non-stop flight and wintering period differentially affected the two astrocytes morphotypes, suggesting distinct physiological roles for these cells. We suggest that morphological changes during the wintering period, may be part of the adaptive plasticity of the local hippocampal circuits of A. interpres in preparation for the long journey back to their breeding sites in the north hemisphere.
