Autor(es): Magalh?es, Fernanda do Carmo ; Resende, Samira Diniz ; Senra, Carolina ; Graeff-Teixeira, Carlos ; Enk, Martin Johannes ; Coelho, Paulo Marcos Zech ; Oliveira, Edward ; Corr?a, Deborah Aparecida Negr?o ; Geiger, Stefan Michael ; Carneiro, Mari?ngela
Data: 2020
Origem: Oasisbr
Assunto(s): Esquistossomose / parasitologia; Esquistossomose / Diagn?stico; Esquistossomose mansoni / parasitologia; Rea??o em Cadeia da Polimerase em Tempo Real / m?todos; Carga Parasit?ria
Universidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.
Funda??o Oswaldo Cruz. Instituto Ren? Rachou. Pesquisa Cl?nica e Pol?ticas P?blicas em Doen?as Infecciosas e Parasit?rias. Belo Horizonte, MG, Brazil.
Universidade Federal do Esp?rito Santo. Centro de Ci?ncias da Sa?de. N?cleo de Doen?as Infecciosas. Vit?ria, ES, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Funda??o Oswaldo Cruz. Instituto Ren? Rachou. Diagn?stico e Terapia de Doen?as Infecciosas e Oncol?gicas. Belo Horizonte, MG, Brazil.
Funda??o Oswaldo Cruz. Instituto Ren? Rachou. Pesquisa Cl?nica e Pol?ticas P?blicas em Doen?as Infecciosas e Parasit?rias. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.
Universidade Federal de Minas Gerais. Instituto de Ci?ncias Biol?gicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil.
Due to the efforts to control schistosomiasis transmission in tropical countries, a large proportion of individuals from endemic areas present low parasite loads, which hinders diagnosis of intestinal schistosomiasis by the Kato-Katz (KK) method. Therefore, the development of more sensitive diagnostic methods is essential for efficient control measures. The aim was to evaluate the accuracy of a real-time polymerase chain reaction (RT-PCR) to detect Schistosoma mansoni DNA in fecal samples of individuals with low parasite loads. A cross-sectional population-based study was conducted in a rural community (n = 257) in Brazil. POC-CCA? was performed in urine and feces were used for RT-PCR. In addition, fecal exams were completed by 18 KK slides, saline gradient and Helmintex techniques. The combined results of the three parasitological tests detected schistosome eggs in 118 participants (45.9%) and composed the consolidated reference standard (CRS). By RT-PCR, 117 out of 215 tested samples were positive, showing 91.4% sensitivity, 80.2% specificity and good concordance with the CRS (kappa = 0.71). RT-PCR identified 86.9% of the individuals eliminating less than 12 eggs/g of feces, demonstrating much better performance than POC-CCA? (50.8%). Our results showed that RT-PCR is a valuable alternative for the diagnosis of intestinal schistosomiasis in individuals with very low parasite loads.
