Author(s):
Cruz, Ana Cec?lia Ribeiro ; Nunes Neto, Joaquim Pinto ; Silva, Sandro Patroca da ; Silva, Eliana Vieira Pinto da ; Pereira, Glennda Juscely Galv?o ; Santos, Maissa Maia ; Monteiro, Hamilton Ant?nio de Oliveira ; Santos, Flavia Barreto dos ; Guimar?es, Ricardo Jos? de Paula Souza e ; Arag?o, Carine Fortes ; Martins, L?via Car?cio
Date: 2020
Origin: Oasisbr
Subject(s): Aedes / anatomia & histologia; Culex / virologia; V?rus Chikungunya / patogenicidade; Mosquitos Vetores / virologia; Genoma Viral; Gen?tipo da ?frica do Centro-Leste-Sul-Africano; Gen?tipo; Vigil?ncia Entomol?gica; Xinguara (PA)
Description
Instituto Evandro Chagas/Minist?rio da Sa?de and to Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) grant number 302462/2018-0
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Universidade do Estado do Par?. Departamento de Patologia. Bel?m, PA, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Funda??o do Instituto Oswaldo Cruz. Laborat?rio de Imunologia Viral. Rio de Janeiro, RJ, Brazil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Programa de P?s-Gradua??o em Virologia. Ananindeua, PA, Brasil.
Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil.
Chikungunya virus (CHIKV) was first reported in Brazil in 2014 and, after it spread countrywide, an outbreak of febrile illness with reports of arthralgia happened in the municipality of Xinguara, Par?, Brazil in 2017, indicating the virus? circulation. Here, we aimed to investigate CHIKV in mosquito vectors collected during an active surveillance of virus isolation in cell culture by using molecular detection and viral genome sequencing. A total of 492 Aedes, Culex and Mansonia mosquitoes were collected and separated in 36 pools according to the species and sex, and 22.2% (8/36) were positive. CHIKV was indentified in pools of Ae. aegypti females (n = 5), an Ae. aegypti male (n = 1) and in Culex quinquefasciatus females (n = 2). However, as the mosquitoes? whole bodies were macerated and used for detection, one cannot suggest the role of the latter in the viral transmission. Despite this, vector competence studies must be carried out in the different species to investigate long-term adaptations. Viral genome sequencing has characterized the East-Central-South-African (ECSA) genotype in all positive pools analyzed, corroborating previous reports for the Amazon region.
